Expression of transforming growth factor beta isoform mRNA in injured peritoneum that healed with adhesions and without adhesions and in uninjured peritoneum

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Expression of transforming growth factor beta isoform mRNA in injured peritoneum that healed with adhesions and without adhesions and in uninjured peritoneum Michael L Freeman, M.D., Ghassan M Saed, Ph.D., Eslam F Elhammady, M.D., Michael P Diamond, M.D. Fertility and Sterility Volume 80, Pages 708-713 (September 2003) DOI: 10.1016/S0015-0282(03)00770-2

FIGURE 1 A representative multiplex RT/PCR gel showing TGF-β1, TGF-β2, and TGF-β3 with β-actin as a housekeeping gene. Normal cecum peritoneum (lane 1), abraded cecum healed without adhesions (lane 2), abraded cecum healed with adhesions (lane 3). Freeman. TGF-β isoform characterization. Fertil Steril 2003. Fertility and Sterility 2003 80, 708-713DOI: (10.1016/S0015-0282(03)00770-2)

FIGURE 2 Multiplex RT-PCR analysis of TGF-β1 mRNA transcripts in peritoneal adhesions and normally healed peritoneum after surgical abrasion and uninjured control peritoneum. The RT-PCR analysis of β-actin and TGF-β1 used total RNA isolated from tissues snap-frozen on the day of necropsy. The PCR products were analyzed on a 2% agarose gel and visualized by ethidium bromide staining. NIH ImageJ (version 1.24t) scanning densimeter software was used to determine the ratio of integrated intensity of each target product band to its respective β-actin control. Error bars represent the SE of the mean of triplicates. *P<.05 against “Healed Normally” and “Control.” Freeman. TGF-β isoform characterization. Fertil Steril 2003. Fertility and Sterility 2003 80, 708-713DOI: (10.1016/S0015-0282(03)00770-2)

FIGURE 3 Multiplex RT-PCR analysis of TGF-β2 mRNA transcripts in peritoneal adhesions and normally healed peritoneum after surgical abrasion and uninjured control peritoneum. The RT-PCR analysis of β-actin and TGF-β2 used total RNA isolated from tissues snap-frozen on the day of necropsy. The PCR products were analyzed on a 2% agarose gel and visualized by ethidium bromide staining. NIH ImageJ (version 1.24t) scanning densimeter software was used to determine the ratio of integrated intensity of each target product band to its respective β-actin control. Error bars represent the SE of the mean of triplicates. Freeman. TGF-β isoform characterization. Fertil Steril 2003. Fertility and Sterility 2003 80, 708-713DOI: (10.1016/S0015-0282(03)00770-2)

FIGURE 4 Multiplex RT-PCR analysis of TGF-β3 mRNA transcripts in peritoneal adhesions and normally healed peritoneum after surgical abrasion and uninjured control peritoneum. The RT-PCR analysis of β-actin and TGF-β2 used total RNA isolated from tissues snap-frozen on the day of necropsy. The PCR products were analyzed on a 2% agarose gel and visualized by ethidium bromide staining. NIH ImageJ (version 1.24t) scanning densimeter software was used to determine the ratio of integrated intensity of each target product band to its respective β-actin control. Error bars represent the SE of the mean of triplicates. †P<.01 against “Healed Normally” and “Control.” Freeman. TGF-β isoform characterization. Fertil Steril 2003. Fertility and Sterility 2003 80, 708-713DOI: (10.1016/S0015-0282(03)00770-2)