Regulation of insulin-like growth factor binding protein secretion by human granulosa luteal cells in a polycystic ovary-like environment  Susanne Greisen, Ph.D., Allan Flyvbjerg, D.M.Sc., Thomas Ledet, D.M.Sc., Per Ovesen, D.M.Sc.  Fertility and Sterility  Volume 78, Issue 1, Pages 162-168 (July 2002) DOI: 10.1016/S0015-0282(02)03147-3

FIGURE 1 Effects of insulin, LH, and elevated androgen (10−5 mol/L) on accumulation of IGFBP-1 through IGFBP-4 in granulosa luteal cell-conditioned medium containing A (10−6 mol/L) and analyzed by WLB. Control conditions in lane 1. Lane 2 shows the effect of insulin (400 μU/mL). Lane 3 illustrates the influence of LH (10 μg/L). Lane 4 shows the result of androgen (10−5 mol/L) addition. Lane 5 shows the result of the addition of the three hormones combined (insulin, 400 μU/mL; LH, 10 μg/L; and A, 10−5 mol/L). Greisen. Effect of PCOS-like environment on IGFBPs. Fertil Steril 2002. Fertility and Sterility 2002 78, 162-168DOI: (10.1016/S0015-0282(02)03147-3)

FIGURE 2 The effects of insulin, LH, and A on the accumulation of IGFBP-1 in granulosa luteal cell-conditioned medium. The light gray columns illustrate the inhibitory effect of insulin (100 μU/mL–800 μU/mL). The dark gray columns show the effect of LH (1 μg/L–10 μg/L), and the solid columns represent the inhibitory consequence of A (10−5 mol/L) on the IGFBP-1 release from human granulosa luteal cells. The striped column illustrates the inhibitory effect of the three hormones combined (insulin, 400 μU/mL; LH, 10 μg/L; and A, 10−5 mol/L). Each column is displayed as mean ± SEM of three independent experiments, with two replicates per trial. ∗IGFBP-1 production differs significantly from control (P<.01). Greisen. Effect of PCOS-like environment on IGFBPs. Fertil Steril 2002. Fertility and Sterility 2002 78, 162-168DOI: (10.1016/S0015-0282(02)03147-3)

FIGURE 3 The effects of insulin, LH, and A on the accumulation of IGFBP-2 in granulosa luteal cell-conditioned medium. The light gray columns illustrate the stimulatory effect of insulin (100 μU/mL–800 μU/mL). The solid columns represent the stimulatory consequence of A (10−5 mol/L) on the IGFBP-2 release from human granulosa luteal cells, and the dark gray columns show that LH (1 μg/L–10 μg/L) does not influence the accumulation in the medium. The striped column illustrates the inhibitory effect of the three hormones combined (insulin 400 μU/mL, LH 10 μg/L, and A 10−5 mol/L) Each column is displayed as mean ± SEM of three independent experiments with two replicates per trial. ∗IGFBP-1 production differs significantly from control (P<.01). Greisen. Effect of PCOS-like environment on IGFBPs. Fertil Steril 2002. Fertility and Sterility 2002 78, 162-168DOI: (10.1016/S0015-0282(02)03147-3)

FIGURE 4 Identification of IGFBP-1 through IGFBP-4 in granulosa luteal cell-conditioned medium containing A (10−6 mol/L) analyzed by Western ligand blotting. Basic conditions in lane 1. Lanes 2–4 represent the effect of insulin (100 μU/mL–800 μU/mL). Lane 5–6 illustrates the effect of LH (1 μg/L–10 μg/L). Greisen. Effect of PCOS-like environment on IGFBPs. Fertil Steril 2002. Fertility and Sterility 2002 78, 162-168DOI: (10.1016/S0015-0282(02)03147-3)