Use of a modified intracytoplasmic sperm injection technique to overcome sperm-borne and oocyte-borne oocyte activation failures Jan Tesarik, M.D., Ph.D.,

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Use of a modified intracytoplasmic sperm injection technique to overcome sperm-borne and oocyte-borne oocyte activation failures Jan Tesarik, M.D., Ph.D., Laura Rienzi, B.Sc., Filippo Ubaldi, M.D., Carmen Mendoza, Ph.D., Ermanno Greco, M.D. Fertility and Sterility Volume 78, Issue 3, Pages 619-624 (September 2002) DOI: 10.1016/S0015-0282(02)03291-0

FIGURE 1 Modified ICSI procedure. (A), Culmination of the ooplasmic aspiration phase. The needle tip is located in the center of the oocyte, and the distance of the sperm head (arrow), marking the front of the aspirated ooplasm column, from the outer surface of the zona pellucida is equal to the distance between the outer surface of the zona pellucida and the oocyte center. (B), Culmination of the ooplasmic reinjection phase. The needle tip is located in the oocyte periphery opposite to the puncture site. The sperm head (arrow) is visible at the needle tip. Tesarik. Overcoming oocyte activation failure. Fertil Steril 2002. Fertility and Sterility 2002 78, 619-624DOI: (10.1016/S0015-0282(02)03291-0)