Elevation of protein levels of O-GlcNAcase in pre-diabetic and diabetic erythrocytes. Elevation of protein levels of O-GlcNAcase in pre-diabetic and diabetic.

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Elevation of protein levels of O-GlcNAcase in pre-diabetic and diabetic erythrocytes. Elevation of protein levels of O-GlcNAcase in pre-diabetic and diabetic erythrocytes. Protein levels of O-GlcNAcase were determined by Western blotting in NIDDK samples (A and B) and JH Center samples (C and D). A: Representative Western blot analysis showing that protein levels of O-GlcNAcase are elevated twofold in the pre-diabetic (n = 13, **P < 0.01) and diabetic samples (n = 9, P < 0.01) compared with normal samples (n = 13). B: Quantitation of Western blot analysis (A). C: Representative Western blot analysis showing that protein levels of O-GlcNAcase are elevated 2.5-fold in the diabetic (n = 14, P < 0.01) compared with normal samples (n = 8). D: Quantitation of Western blot analysis (C). Actin was used as a loading control. E: Protein levels of O-GlcNAcase were estimated in JH Center samples by Western blotting with recombinant O-GlcNAcase as standard. F: Quantitation based on Western blot analysis. Diabetic samples (18.70 ± 5.91 ng/100 μg of proteins, n = 10, *P < 0.05) have threefold more O-GlcNAcase than normal samples (6.10 ± 1.93 ng/100 μg of proteins, n = 10). Data are means ± SEM. Kyoungsook Park et al. Diabetes 2010;59:1845-1850 ©2010 by American Diabetes Association