Blastocyst gene expression correlates with implantation potential

Slides:

Advertisements

Similar presentations

Molecular characterization of uterine fibroids and its implication for underlying mechanisms of pathogenesis Paul J. Hoffman, B.S., Dawn B. Milliken,

Advertisements

A review and meta-analysis of prospective trials comparing different catheters used for embryo transfer William M. Buckett, M.D. Fertility and Sterility

Demián Glujovsky, M.D., M.Sc., Cynthia Farquhar, M.D., M.P.H.

Validation of microarray comparative genomic hybridization for comprehensive chromosome analysis of embryos Cristina Gutiérrez-Mateo, Ph.D., Pere Colls,

Nick Macklon, M.D., Ph.D. Fertility and Sterility

MicroRNAs in spent blastocyst culture medium are derived from trophectoderm cells and can be explored for human embryo reproductive competence assessment

Adenomyosis does not affect implantation, but is associated with miscarriage in patients undergoing oocyte donation José A. Martínez-Conejero, Ph.D.,

Ratio between inner cell mass diameter and blastocyst diameter is correlated with successful pregnancy outcomes of single blastocyst transfers Miriam.

Soyoung Bae, M.D., Joseph Karnitis, M.D. Fertility and Sterility

Potential role of circulating microRNAs as a biomarker for unexplained recurrent spontaneous abortion Weibing Qin, M.D., Ph.D., Yunge Tang, M.D., Ning.

Gene expression profiling of multiple leiomyomata uteri and matched normal tissue from a single patient Irina K. Dimitrova, M.D., Jennifer K. Richer,

Gene expression profiling of human peri-implantation endometria between natural and stimulated cycles Yunao Liu, M.Sc., Kai-Fai Lee, Ph.D., Ernest H.Y.

Corona cell RNA sequencing from individual oocytes revealed transcripts and pathways linked to euploid oocyte competence and live birth Jason C. Parks,

Michael M. Alper, M.D. Fertility and Sterility

Agnieszka Malcher, M. S. , Natalia Rozwadowska, Ph. D

Single-gene testing combined with single nucleotide polymorphism microarray preimplantation genetic diagnosis for aneuploidy: a novel approach in optimizing.

Can an educational DVD improve the acceptability of elective single embryo transfer? A randomized controlled study Nicole Hope, M.B., B.S., Hon., Luk.

Mohamad Irani, M. D. , David Reichman, M. D. , Alex Robles, M. D

Chun Feng, M. D. , Shen Tian, Ph. D. , Yu Zhang, M. D. , Jing He, M. D

Medical treatment of ectopic pregnancy: a committee opinion

The relative contribution of assisted reproductive technologies and ovulation induction to multiple births in the United States 5 years after the Society.

Embryology in the era of proteomics

Iris G. Insogna, M. D. , Malinda S. Lee, M. D. , M. B. A. , Rebecca M

Optimizing the number of blastocyst stage embryos to transfer on day 5 or 6 in women 38 years of age and older: a Society for Assisted Reproductive Technology.

Age-associated alteration of oocyte-specific gene expression in polar bodies: potential markers of oocyte competence Ze-Xu Jiao, M.D., Ph.D., Min Xu,

Proteomic analysis of individual human embryos to identify novel biomarkers of development and viability Mandy G. Katz-Jaffe, Ph.D., David K. Gardner,

MicroRNA expression profiles in human testicular tissues of infertile men with different histopathologic patterns Masood Abu-Halima, M.Sc., Christina.

Noninvasive metabolomic profiling as an adjunct to morphology for noninvasive embryo assessment in women undergoing single embryo transfer Emre Seli,

Does endometrial integrin expression in endometriosis patients predict enhanced in vitro fertilization cycle outcomes after prolonged GnRH agonist therapy?

Dana B. McQueen, M. D. , M. A. S. , Candice O. Perfetto, M. D

Reply of the Authors Fertility and Sterility

A comparison of three downregulation approaches for poor responders undergoing in vitro fertilization Laura Detti, M.D., Daniel B. Williams, M.D., Jared.

Optimizing the culture environment and embryo manipulation to help maintain embryo developmental potential Jason E. Swain, Ph.D., Doug Carrell, Ph.D.,

High relative deoxyribonucleic acid content of trophectoderm biopsy adversely affects pregnancy outcomes Shelby A. Neal, M.D., Jason M. Franasiak, M.D.,

Imprinting disorders and assisted reproductive technology

Patricia Díaz-Gimeno, Ph. D. , José A. Horcajadas, Ph. D. , José A

Mandy G. Katz-Jaffe, Ph. D. , Mark G. Larman, Ph. D. , Courtney B

Management of tubal ectopic pregnancy: methotrexate and salpingostomy are preferred to preserve fertility Stephanie Beall, M.D., Ph.D., Alan H. DeCherney,

Modulation of HOXA10 and other markers of endometrial receptivity by age and human chorionic gonadotropin in an endometrial explant model Robin H. Fogle,

The endometrial receptivity array for diagnosis and personalized embryo transfer as a treatment for patients with repeated implantation failure Maria.

Should embryos developing to blastocysts on day 7 be cryopreserved and transferred: an analysis of pregnancy and implantation rates George Kovalevsky,

Selection of viable spermatozoa from testicular biopsies: a comparative study between pentoxifylline and hypoosmotic swelling test Vijay Mangoli, Ph.D.,

Akanksha Mehta, M.D., Darius A. Paduch, M.D., Ph.D.

Comprehensive chromosome screening of polar bodies and blastocysts from couples experiencing repeated implantation failure Elpida Fragouli, Ph.D., Mandy.

Samer Alfarawati, M. S. , Elpida Fragouli, Ph. D. , Pere Colls, Ph. D

Number of good quality embryos on day 3 is predictive for both pregnancy and implantation rates in in vitro fertilization/intracytoplasmic sperm injection.

“Information-rich” reproductive outcomes in carriers of a structural chromosome rearrangement ascertained on the basis of recurrent pregnancy loss Michelle.

Aaron K. Styer, M. D. , Diane L. Wright, Ph. D. , H. C. L. D. , Anne M

William B. Schoolcraft, M. D. , Elpida Fragouli, Ph. D

Cameron Gilbert, M. Sc. , Maria Valois, M. D. , FRCPC, Ph. D

Human myometrium and leiomyomas express gonadotropin-releasing hormone 2 and gonadotropin-releasing hormone 2 receptor Jason D. Parker, D.O., Minnie.

Pregnancy outcome in infertile patients with polycystic ovary syndrome who were treated with metformin Samuel S. Thatcher, M.D., Ph.D., Elizabeth M.

Molecular profiling of experimental endometriosis identified gene expression patterns in common with human disease Idhaliz Flores, Ph.D., Elizabeth Rivera,

A mandatory single blastocyst transfer policy with educational campaign in a United States IVF program reduces multiple gestation rates without sacrificing.

Reducing the dose of human chorionic gonadotropin in high responders does not affect the outcomes of in vitro fertilization David W. Schmidt, M.D.,,

Amy Criniti, M. D. , Angela Thyer, M. D. , Gregory Chow, M. D

Ryan K. C. Yuen, Ph. D. , Anna Merkoulovitch, B. CS. , Jeffrey R

Failed IVF cycles and the risk of subsequent preeclampsia or fetal growth restriction: a case-control exploratory study Ioanna Tsoumpou, M.D., Ahmed.

Eric S. Surrey, M. D. , Debra A. Minjarez, M. D. , John M. Stevens, B

Assessing the reproductive competence of individual embryos: a proposal for the validation of new “-omics” technologies Richard T. Scott, M.D., H.C.L.D.,

Susanna McReynolds, Ph. D. , Monika Dzieciatkowska, Ph. D

A review and meta-analysis of prospective trials comparing different catheters used for embryo transfer William M. Buckett, M.D. Fertility and Sterility

Blastocyst biopsy with comprehensive chromosome screening and fresh embryo transfer significantly increases in vitro fertilization implantation and delivery.

Endometrial gene expression in the window of implantation is altered in obese women especially in association with polycystic ovary syndrome José Bellver,

Adrienne B. Neithardt, M. D. , James H. Segars, M. D

Endometrial polyps affect uterine receptivity

Susanna McReynolds, Ph. D. , Monika Dzieciatkowska, Ph. D. , Blair R

Preimplantation genetic screening: what is the clinical efficiency?

Akash A. Kapadia, M. D. , Marcus Anthony, B. Sc

Male age negatively impacts embryo development and reproductive outcome in donor oocyte assisted reproductive technology cycles John L. Frattarelli,

Presentation transcript:

Blastocyst gene expression correlates with implantation potential Jason C. Parks, B.Sc., Blair R. McCallie, B.Sc., Ann M. Janesch, B.Sc., William B. Schoolcraft, M.D., Mandy G. Katz-Jaffe, Ph.D. Fertility and Sterility Volume 95, Issue 4, Pages 1367-1372 (March 2011) DOI: 10.1016/j.fertnstert.2010.08.009 Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Individual blastocysts of equivalent morphology were transferred after TE biopsy. Q RT-PCR on individual biopsied samples revealed differential gene expression profiles corresponding to implantation outcome. Positive implantation with healthy fetal development displayed increased expression of B3gnt5, Eomes, and Wnt3a (∗P<.05). Nonviable implantation or pregnancy loss showed significant decrease in gene expression of B3gnt5 and Wnt3a (∗P<.05). Negative implantation revealed a decrease in gene expression of B3gnt5 and Eomes (∗P<.05). Fertility and Sterility 2011 95, 1367-1372DOI: (10.1016/j.fertnstert.2010.08.009) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 2 (A) Unsupervised hierarchical clustering of all microarray samples classified two distinct transcriptomes with complete separation of healthy placental samples from biopsied absorption sites. Normalized gene expression ratios are depicted by color intensity, with the highest expression corresponding to red and the lowest expression corresponding to blue. (B) Validation of microarray data using Q RT-PCR examined the expression of 10 altered complement and coagulation cascade genes relative to the housekeeping gene, Gapdh. Differential expression was validated for 9 of the 11 genes analyzed (∗P<.05). Fertility and Sterility 2011 95, 1367-1372DOI: (10.1016/j.fertnstert.2010.08.009) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions