Effects of Chd1-knockdown on Hmgpi and Klf5 expression

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Effects of Chd1-knockdown on Hmgpi and Klf5 expression Effects of Chd1-knockdown on Hmgpi and Klf5 expression. qRT-PCR analyses of (A) Hmgpi and (C) Klf5 mRNA in control and Chd1-knockdown embryos from the early 2-cell to the blastocyst stages (*P<0.05). Effects of Chd1-knockdown on Hmgpi and Klf5 expression. qRT-PCR analyses of (A) Hmgpi and (C) Klf5 mRNA in control and Chd1-knockdown embryos from the early 2-cell to the blastocyst stages (*P<0.05). Expression levels were normalized to H2afz, used as an internal control. The mRNA levels of the late 2-cell control embryos were defined as 1. Data are expressed as means±s.e.m. (n=3). (B) Immunofluorescence detection of HMGPI in control and Chd1-knockdown embryos (green, HMGPI; blue, chromatin). Representative images of embryos at each stage are shown. Between 20 and 30 embryos were used in each experiment (n=3): in total, 59, 75, 95 and 102 control embryos, and 76, 82, 75 and 114 Chd1-knockdown embryos each for 4-cell, 8-cell, morula and blastocyst stage, respectively. (D) Immunofluorescence detection of KLF5 in control and Chd1-knockdown embryos (green, KLF5; blue, chromatin). Representative images of embryos at each stage are shown. Between 20 and 50 embryos were used in each experiment (n=3): in total, 87, 59, 117 and 126 control embryos, and 90, 76, 122 and 144 Chd1-knockdown embryos each for late 2-cell, 4-cell, 8-cell, morula and blastocyst stage, respectively. Shinnosuke Suzuki et al. Development 2015;142:2375-2384 © 2015. Published by The Company of Biologists Ltd