Identification of differential gene expression in germinal vesicle vs

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Identification of differential gene expression in germinal vesicle vs Identification of differential gene expression in germinal vesicle vs. metaphase II mouse oocytes by using annealing control primers Se-Jin Yoon, M.Sc., Hyung-Min Chung, Ph.D., Kwang-Yul Cha, M.D., Nam-Hyung Kim, Ph.D., Kyung-Ah Lee, Ph.D. Fertility and Sterility Volume 83, Issue 4, Pages 1293-1296 (April 2005) DOI: 10.1016/j.fertnstert.2004.09.037 Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 Differentially expressed genes of germinal vesicle (GV) and MII oocytes. (A) Representative GV and MII oocytes. (B) Representative band patterns for differential expression between GV and MII oocytes after annealing control primer-polymerase chain reaction (ACP-PCR). Messenger RNA was isolated from equal numbers of oocytes and subjected to reverse transcription-polymerase chain reaction (RT-PCR) using ACP. The PCR products were separated by electrophoresis on 2% agarose gels. M, lane containing a 100-bp DNA ladder. (C) Quantitative real-time PCR analysis with gene-specific primers to confirm differential expression. The CT values are as follows: CSN3 (GV = 20.91; MII = 27.92), PKD2 (GV = 23.16; MII = 32.40), and Pscd2 (GV = 24.27; MII = 35.44). (D) Real-time PCR products were subjected to 1.5% agarose gel separation for further confirmation. Rabbit α-globin mRNA served as an external standard. Fertility and Sterility 2005 83, 1293-1296DOI: (10.1016/j.fertnstert.2004.09.037) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions