Characterization of iPSC-derived eye field primordial (EFP) clusters.

Slides:

Advertisements

Similar presentations

DEVELOPMENT OF EYE. Eyes are derived from four sources Neuroectoderm of fore brain retina, posterior layers of iris, optic nerve Surface ectoderm of head.

Advertisements

FIGURE 22.1 Isolation of Organoid Structures from Breast. (a) Flowchart of isolation of different fractions by selective sedimentation and centrifugation.

Shiva Swamynathan Eye Development and Gene Expression.

Psychology 304: Brain and Behaviour Lecture 28

Invest. Ophthalmol. Vis. Sci ;50(2): doi: /iovs Figure Legend:

From: Fate Maps of Neural Crest and Mesoderm in the Mammalian Eye

From: Polarized Secretion of PEDF from Human Embryonic Stem Cell–Derived RPE Promotes Retinal Progenitor Cell Survival Invest. Ophthalmol. Vis. Sci ;52(3):

From: Zeb1 Represses Mitf and Regulates Pigment Synthesis, Cell Proliferation, and Epithelial Morphology Invest. Ophthalmol. Vis. Sci ;50(11):

Development of the eye:

Primary culture of mouse middle ear epithelial cells.

Reinventing a Common Strategy for Patterning the Eye

Corneal Epithelial Stem Cells: Past, Present, and Future

Ocular defects produced by Daam1 mutation and in combination with diabetes. Ocular defects produced by Daam1 mutation and in combination with diabetes.

Keeping in touch with contact inhibition of locomotion

Volume 10, Issue 1, Pages (January 2018)

The neuroepithelium of mid-neurulation mammalian embryos displays planar-polarised F-actin profiles. The neuroepithelium of mid-neurulation mammalian embryos.

Scanning electron microscopy analysis of EGK-I to -V chick embryos.

Salmonella enterica serovar typhimurium (S typhimurium) TML invades human terminal ileal epithelial tissue. Salmonella enterica serovar typhimurium (S.

José María Frade, Yves-Alain Barde Neuron

Progression of a drusenoid RPE detachment to choroidal neovascularisation (CNV) over a 6 year period. Progression of a drusenoid RPE detachment to choroidal.

The retinal pigmented epithelium is required for development and maintenance of the mouse neural retina Sophie M. Raymond, Ian J. Jackson Current Biology

(case 6) (A) Fundus photography showing subtle discrete areas of RPE atrophy (green areas). (case 6) (A) Fundus photography showing subtle discrete areas.

Neural Regeneration and Cell Replacement: A View from the Eye

Volume 148, Issue 1, (January 2012)

Single colour fundus photographs of pseudopapilloedema in patients initially thought to have IIH. (A) Elevated, lumpy disc with anomalous vascular pattern.

Orientation defects in stereociliary hair bundles with uncoupling from kinocilium and basal body position of hair cells in the organ of Corti of neonatal.

Naive and Primed Pluripotent States

Volume 8, Issue 4, Pages (April 2005)

Neovascularization, macular edema and retinal detachments in the vhl−/− retina. Neovascularization, macular edema and retinal detachments in thevhl−/−retina.

Fig. 8. In vitro effect of CMPs on adult DSS-treated stomach explants.

BTN1 mRNA level in response to extracellular pH determined by comparative RT2PCR. BTN1mRNA level in response to extracellular pH determined by comparative.

Localisation of epithelial markers in mMEC cultures.

A subset of Trp53CKO fiber cells fail to exit the cell cycle, but express fiber-cell-specific markers. A subset of Trp53CKO fiber cells fail to exit the.

X-ray radiographic images showing structural alterations of the joints that are most commonly affected by OA. (A) Normal and (A′) severely affected joint.

Embryonic corneal defects in E18.5 AP-2β NCC KO mutant embryos.

Fig. 5. Onecut transcription factors are important for the correct generation of the mdDA neuronal population.(A) Schematic representation of the region.

Fig. 1. Morphological and growth characterization of hBMCs and hPDCs

Fig. 4. The model of malate metabolism in fruit cells under different K level conditions. The model of malate metabolism in fruit cells under different.

Fig. 1. γ-Tubulin localizes in close proximity to centriole walls in interphase but within an extended PCM meshwork in mitosis.U2OS cells were fixed and.

Differentiation of neural crest cells into corneal endothelial cells

Extended micro-organoid culture.

Fig. 3. Low power hemi retinal image of the RPE surface showing the albino central and equatorial retina and pigment distribution in the periphery. Low.

Fig. 5. The bulk of Cep135 localizes distantly from Sas-6 and STIL

Fig. 2. Morphological analysis of acentriolar mitotic spindles

Fig. 2. Blocking of BMP signaling, but not of nodal signaling, inhibits autochthonous neural crest migration in zebrafish embryos. Blocking of BMP signaling,

Co-development of epithelium and vasculature in early developing submandibular gland. Co-development of epithelium and vasculature in early developing.

Fig. 8. Lhx1-RNAi perturbs NR formation

Fig. 5. UV reflections from the eye cup.

Loss of SOX2 in K14+ progenitors blocks fate acquisition of taste and non-taste epithelial cells. Loss of SOX2 in K14+ progenitors blocks fate acquisition.

Fig. 7. Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and.

HSP90β interacts with HNF4A to regulate protein half-life.

Fig. 3. Immunostaining for neural differentiation makers of the stage 29 optic cup.Immunoreactive signals are shown in green or red. Immunostaining for.

Abnormally low or high Tj expression causes a decrease in DEcad expression and shg enhancer activity. Abnormally low or high Tj expression causes a decrease.

Fig. 3. Exogenous folic acid rescues neural epithelial apical constriction and activation of non-muscle myosin upon Rho-kinase inhibition. Exogenous folic.

Effect of Dipy on the expression of chondrogenic markers.

Fig. 2. iPSCs produce functional osteoblasts.

Self-organization into organoids, gastruloids and embryoids.

Prophase nuclear movements in wild-type, rec8 and rec7 mutant cells.

Organoid-derived transplantable corneal epithelial grafts on human amniotic membrane substrate. Organoid-derived transplantable corneal epithelial grafts.

IGF1R is present in endometrial luminal epithelium at the receptive phase. IGF1R is present in endometrial luminal epithelium at the receptive phase. Endometrial.

IHC characterization of 10- to 15-week-old adherent MCs

IHC characterization of 15-week-old mature corneal organoid in suspension. IHC characterization of 15-week-old mature corneal organoid in suspension. Confocal.

The expression of two forms of N-cadherin.

Computational modeling input and results.

Fig. 1. Generation of induced pluripotent stem cells (iPSCs) from urine cells (UC). Generation of induced pluripotent stem cells (iPSCs) from urine cells.

José María Frade, Yves-Alain Barde Neuron

Depletion of COPI protein inhibits cis to trans cisternal maturation.

Adult Acvr1;Trp53DCKO lenses form vascularized, tumor-like structures at their posterior poles. 4-month-old Trp53CKO and Acvr1;Trp53DCKO lenses were sectioned.

Dynamic and polarized recruitment of ERC1a near protrusive sites during migration. Dynamic and polarized recruitment of ERC1a near protrusive sites during.

Effect of myosin Vb shRNA on ezrin phosphorylation and microvilli organization in Caco-2 cells. Effect of myosin Vb shRNA on ezrin phosphorylation and.

Presentation transcript:

Characterization of iPSC-derived eye field primordial (EFP) clusters. Characterization of iPSC-derived eye field primordial (EFP) clusters. (A) Schematic representation of the stepwise differentiation of human iPSCs into retinal and corneal organoids. Growing iPSCs are first differentiated into EFPs, which upon isolation and suspension culture give rise to both retinal primordia (RP) and corneal primordia (CP). The dissected-out CP are cultured under corneal differentiation conditions for further maturation. CDM, corneal differentiation medium; RDM, retinal differentiation medium; RPE, retinal pigmented epithelium. (B) Distinct circular to oval-shaped EFPs encompassed a centrally located CHX10+ RCVRN+ neuroretinal (NR) cup (asterisks) (i). Pigmented neural crest-derived cells and P63+ ocular surface ectodermal (OSE) cells appear to differentiate from the edges of EFP clusters. Arrows point to a distinct margin comprising a spindle-shaped melanocyte enriched-zone between the NR and OSE cells (i-iv). Arrowheads point to crystallin+ lentoid clusters adjacent to NR cups (v) and a phase image showing the presence of pigmented melanocytes (arrows) over a layer of epithelial sheet within the migratory cell zone (vi). Praveen Joseph Susaimanickam et al. Development 2017;144:2338-2351 © 2017. Published by The Company of Biologists Ltd