Inhibition of allergen-specific IgE reactivity by a human Ig Fcγ-Fcε bifunctional fusion protein Ke Zhang, MD, PhD, Christopher L Kepley, PhD, Tetsuya.

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Presentation transcript:

Inhibition of allergen-specific IgE reactivity by a human Ig Fcγ-Fcε bifunctional fusion protein Ke Zhang, MD, PhD, Christopher L Kepley, PhD, Tetsuya Terada, MD, PhD, Daocheng Zhu, MD, PhD, Hector Perez, MD, Andrew Saxon, MD Journal of Allergy and Clinical Immunology Volume 114, Issue 2, Pages 321-327 (August 2004) DOI: 10.1016/j.jaci.2004.03.058

Fig 1 Diagram of GE2 fusion protein and the proposed mechanism by which it inhibits FcεRI-mediated functions. A, Schematic diagram of GE2 construct. B, Ribbon drawing for the 3-dimensional structure of the dimerized GE2. C, Proposed mechanism by which GE2 inhibits FcεRI-mediated degranulation.7 The tetrameric αβγ2 FcεRI receptor normally is activated by crosslinking of the IgE-primed FcεRI with multivalent antigen, resulting in the activation of Lyn, which in turn phosphorylates ITAMs within the FcεRI and creates docking sites for Syk kinase family members that result in signal propagation, which culminates in mast cell activation and degranulation. GE2, by binding FcεRI and FcγRIIb as shown, cocrosslinks FcγRIIb with FcεRI, resulting in the blocking of IgE-dependent degranulation. This inhibition results from the ITIM activation in FcγRIIb, which leads to the interruption of Syk signaling.7 ITAM, Immunoreceptor tyrosine-based activation motif; ITIM, immunoreceptor tyrosine-based inhibition motif. Journal of Allergy and Clinical Immunology 2004 114, 321-327DOI: (10.1016/j.jaci.2004.03.058)

Fig 2 GE2 inhibits FcεRI-mediated degranulation from basophils obtained from donors with cat allergy. Basophils were incubated with or without GE2 or nonspecific control IgE (PS) in Iscove medium at 37°C. The cells were incubated with or without 100 ng/mL Fel d 1 for 30 minutes, and mediator release was measured in the supernatants. Results are represented as the SEM of 3 experiments from 3 separate donors (± SEM). The asterisk indicates a statistically significant P value of .03. Journal of Allergy and Clinical Immunology 2004 114, 321-327DOI: (10.1016/j.jaci.2004.03.058)

Fig 3 GE2 inhibits FcεRI-mediated degranulation from tissue mast cells. Mast cell–containing lung chunks were incubated with or without GE2 or nonspecific IgE (PS) or IgG in Iscove medium at 37°C. The tissue chunks were incubated with or without 1 μg/mL anti-IgE for 45 minutes, and mediator release was measured in the supernatants. Fresh medium was added back to the tissue, which was freeze-thawed 3 times and centrifuged to obtain the total amount of histamine. Results are represented as the SEM of 2 experiments (± SEM). The asterisk indicates a statistically significant P value of .04. Journal of Allergy and Clinical Immunology 2004 114, 321-327DOI: (10.1016/j.jaci.2004.03.058)

Fig 4 GE2 inhibits Fel d 1–specific IgE-mediated mast cell degranulation in vivo. The skin on the back of FcεRIα transgenic mice was injected with serum (9632) from a patient with cat allergy (1:5 dilution; a); serum with 2 μg of GE2 administered 4 hours later (b); serum with 0.2 μg of GE2 (c); serum with 0.02 μg of GE2 (d); myeloma IgE (PS, 250 ng; e); NP-specific IgE (250 ng; f); heat-inactivated serum 9632 (56°C for 30 minutes; g); or nonallergic serum (h). After another 4 hours, the mice were challenged intravenously with 10 μg of purified Fel d 1 plus 1% Evans blue dye in 200 μL and killed 30 minutes after challenge. The results are representative of 3 experiments with similar results. Journal of Allergy and Clinical Immunology 2004 114, 321-327DOI: (10.1016/j.jaci.2004.03.058)

Fig 5 GE2 inhibits dust mite–induced mast cell degranulation in vivo in rhesus monkeys. Sites 3, 5, and 7 were injected with 250, 125, and 62.5 ng of GE2, whereas sites 4, 6, and 8 were injected with 250, 125, and 62.5 ng of control IgE (PS IgE), respectively. Five hours later, 5 mL of 0.5% Evans blue dye was injected intravenously, and the GE2 and PS IgE-injected spots were challenged with a predetermined dose of D farinae allergen. The skin reaction was recorded 30 minutes after challenge. A, Skin test results from monkey M23A. B, Skin test results from monkey R80. The results are representatives of the 5 monkeys, the results of which are shown in Table I. Journal of Allergy and Clinical Immunology 2004 114, 321-327DOI: (10.1016/j.jaci.2004.03.058)