Measurement of insulin release from islets evoked by glucose, diazoxide, and high K+. Measurement of insulin release from islets evoked by glucose, diazoxide,

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Measurement of insulin release from islets evoked by glucose, diazoxide, and high K+. Measurement of insulin release from islets evoked by glucose, diazoxide, and high K+. Insulin secretion from 10 islets treated with a mixture of glucose (G), diazoxide (Dia) (250 μmol/l), and K+ (30 mmol/l) at 37°C for 60 min was measured. Ordinates, insulin content in the supernatant. Each bar represents the mean + SEM of 7–9 tubes. White and black bars indicate data from WT and TRPM2-KO islets, respectively. Statistical significance was assessed using ANOVA followed by the Bonferroni-type multiple t test. **P < 0.01 for WT versus TRPM2-KO mice. #P < 0.05 versus 3.3 mmol/l G. Kunitoshi Uchida et al. Diabetes 2011;60:119-126 ©2011 by American Diabetes Association