Fig. 3. Parp mutation rescues mitochondrial function in pink1 mutants.

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Fig. 3. Parp mutation rescues mitochondrial function in pink1 mutants. Parp mutation rescues mitochondrial function in pink1 mutants. (A) The levels of oxidative stress-related metabolites are increased in pink1 mutants. The metabolites indicated in red or blue are significantly upregulated or downregulated, respectively, compared to control (P<0.05). ND corresponds to a metabolite below detection threshold. The statistical significance for fold-changes was determined using Welch's two-sample t-test (n=8). (B) Protein PARylation is increased in pink1 mutants, and this increase is attenuated in pink1, ParpCH1/+ double mutants. Whole-fly lysates were analysed using the indicated antibodies. Tubulin was used as a loading control. Ponceau S staining was used to assess total protein load. Ratios of signal intensity between total PAR and total protein load (Ponceau S) are presented. Two biological replicates are shown for each genotype. Red text indicates ratios of samples with high level of PARylation. (C) Parp mutation protects against the loss of Δψm in pink1 mutants (mean±s.d.; asterisks, one-way ANOVA with Bonferroni's multiple comparison test). (D) Parp mutation increases complex I-mediated respiration in pink1 mutants (mean±s.d.; asterisks, one-way ANOVA with Bonferroni's multiple comparison test). Datasets in C and D labelled ‘control’ and ‘ParpCH1/+’ have been previously published in Lehmann et al. (2016), as data from these genotypes were obtained as a single experimental set before statistical analysis. (E,F) Parp mutation rescues mitochondrial cristae fragmentation in pink1 mutant brains. (E) Percentages of neuropile mitochondria exhibiting fragmented cristae normalised to the area are presented for the indicated genotypes (asterisks, two-tailed chi-square test, 95% confidence intervals). Datasets labelled control and pink1 are also used in Fig. 1C. (F) Representative TEM micrographs of the indicated genotypes are shown (m, mitochondria). Genotypes: control: w1118, pink1: pink1B9, pink1, parpCH1/+: pink1B9, ParpCH1/+. Susann Lehmann et al. Biology Open 2017;6:141-147 © 2017. Published by The Company of Biologists Ltd