Induced sputum: Validity of fluid-phase IL-5 measurement

Slides:

Advertisements

Similar presentations

Efficacy and safety overview of a new inhaled corticosteroid, QVAR (hydrofluoroalkane- beclomethasone extrafine inhalation aerosol), in asthma Jennifer.

Advertisements

Reducing relative humidity to control the house dust mite Dermatophagoides farinae Larry G. Arlian, PhD, Jacqueline S. Neal, BS, DiAnn L. Vyszenski-Moher,

Control of airborne latex by use of powder-free latex gloves

Anaphylaxis induced by horsefly bites: Identification of a 69 kd IgE-binding salivary gland protein from Chrysops spp. (Diptera Tabanidae) by western.

W.Travis Cain, MDa, Greg Cable, PhDb, John J. Oppenheimer, MDc

Immune function in cigarette smokers who quit smoking for 31 days

Allergen immunotherapy: A practice parameter third update

Different expression of cytokine and membrane molecules by circulating lymphocytes on acute mental stress in patients with atopic dermatitis in comparison.

The effects of upper respiratory infection on T-cell proliferation and steroid sensitivity of asthmatics Elcio O. Vianna, MD, PhD, Jay Westcott, PhD,

Asthma and increased bronchial responsiveness in elite athletes: Atopy and sport event as risk factors Ilkka J. Helenius, MDa, Heikki O. Tikkanen, MDb,

Rosa Codina, PhD, Richard F. Lockey, MD

Aspirin-induced urticaria and angioedema, but not bronchoconstriction, associated with cysteinyl leukotriene overproduction in 2 patients with asthma

Comparison of once-daily ebastine 20 mg, ebastine 10 mg, loratadine 10 mg, and placebo in the treatment of seasonal allergic rhinitis Paul H. Ratner,

Is 9 more than 2 also in allergic airway inflammation?

Elevated circulating adenosine level potentiates antigen-induced immediate bronchospasm and bronchoconstrictor mediator release in sensitized guinea pigs

Deficient prostaglandin E2 production by bronchial fibroblasts of asthmatic patients, with special reference to aspirin-induced asthma Malgorzata Pierzchalska,

Practice Notes from the AAAI

Badrul A. Chowdhury, MD, PhD

Hydroxychloroquine improves airflow and lowers circulating IgE levels in subjects with moderate symptomatic asthma B.Lauren Charous, MD, Elkan F. Halpern,

Anaphylaxis to ingestion of hempseed (Cannabis sativa)

Product characteristics and pharmacokinetics of intranasal ipratropium bromide Chester C. Wood, MDa, Philip Fireman, MDb, Jay Grossman, MDc, Margaret.

Allergy to eggs from duck and goose without sensitization to hen egg proteins Belén Añíbarro, MD, F.Javier Seoane, MD, Concepción Vila, MD, Manuel Lombardero,

Elephantiasis nostras: A case report

Common allergens in avian meats

Conjunctivitis medicamentosa

Inhaled corticosteroids: Impact on asthma morbidity and mortality

Practice Notes from the AAAAI

Yanli Ouyang, MD, PhDa, Nisha Virasch, BSa, Ping Hao, MDa, Michael T

A review of the current guidelines for allergic rhinitis and asthma

Physician-targeted program on inhaled therapy for childhood asthma

News & Notes Journal of Allergy and Clinical Immunology

Correlation between alcohol-induced asthma and acetaldehyde dehydrogenase-2 genotype Atsuko Takao, MDa, Terufumi Shimoda, MDa, Shigeru Kohno, MDa, Sadahiro.

Circadian variation of sputum inflammatory cells in mild asthma

Inflammatory cells, cytokine and chemokine expression in asthma immunocytochemistry and in situ hybridization Qutayba Hamid, MD, PhD, Editor Journal.

The editors' choice Journal of Allergy and Clinical Immunology

Patterns of pollen cross-allergenicity

Molecular virology and immunology of HIV infection

Jack M. Becker, MDa, Anjali Arora, MDb, Richard J

Vascular endothelial growth factor in patients with acute asthma

News & Notes Journal of Allergy and Clinical Immunology

Corticosteroid-induced apoptosis in mouse airway epithelium: Effect in normal airways and after allergen-induced airway inflammation Delbert R. Dorscheid,

Occupational allergy caused by urticating hair of Brazilian spider

Effect of rush immunotherapy on airway inflammation and airway hyperresponsiveness after bronchoprovocation with allergen in asthma Yasurou Kohno, MDa,

A randomized, double-blind trial of the effect of treatment with montelukast on bronchial hyperresponsiveness and serum eosinophilic cationic protein.

The 30th anniversary of the American Board of Allergy and Immunology: Then and now Lynn Des Prez a, Charles E. Reed, MD, Lawrence B. Schwartz, MDb, John.

Emma Broadfield, MRCP, Tricia M

Ajay B. Antony, HSa, Robert S. Tepper, MDb, Kamal A. Mohammed, PhDa

Loratadine reduces allergen-induced mucosal output of α2-macroglobulin and tryptase in allergic rhinitis Lennart Greiff, MD, PhDa, Carl G.A. Persson,

The role and remediation of animal allergens in allergic diseases

Targeting keratinocyte apoptosis in the treatment of atopic dermatitis and allergic contact dermatitis Axel Trautmann, MDa, Mübeccel Akdis, MDa, Peter.

The effects of IL-5 on airway physiology and inflammation in rats

Preparing patients and health professionals for the transition to chlorofluorocarbon-free inhalers: The British perspective Helen Donohoe, MSc (Econ)

Housing characteristics, reported mold exposure, and asthma in the European Community Respiratory Health Survey Jan-Paul Zock, PhDa, Deborah Jarvis,

Practice Notes from the AAAI

A case of acute eosinophilic pneumonia with hypersensitivity to “red spider” allergens Bertrand Godeau, MDa, Laurent Brochard, MDb, Ioanis Theodorou,

Deposition of fenoterol from pressurized metered dose inhalers containing hydrofluoroalkanes Stephen Newman, PhDa, Gary Pitcairn, PhDa, Karen Steed,

Anaphylaxis after initial ingestion of rambutan, a tropical fruit

Determinants of systemic manifestations of food allergy

Robert P. Schleimer, PhD Journal of Allergy and Clinical Immunology

Terence J. Furlong, MSa, Jennifer DeSimonea, Scott H. Sicherer, MDb

Footnotes1 Journal of Allergy and Clinical Immunology

National trends in asthma visits and asthma pharmacotherapy,

Prescott G. Woodruff, MD, MPH, Ramin Khashayar, MD, Stephen C

Larry C. Borish, MDa, Harold S

Evelyn Farinas, RPh, MGA, Peter Honig, MD

The environmental predictors of allergic disease

Reciprocal regulation of cultured human mast cell cytokine production by IL-4 and IFN-γ Hiroshi Tachimoto, MD, PhDa, Motohiro Ebisawa, MD, PhDa,b, Tomohide.

Airway remodeling-associated mediators in moderate to severe asthma: Effect of steroids on TGF-β, IL-11, IL-17, and type I and type III collagen expression

News & Notes Journal of Allergy and Clinical Immunology

Primary prevention of asthma and allergy

Presentation transcript:

Induced sputum: Validity of fluid-phase IL-5 measurement Margaret M. Kelly, MBChBa, Richard Leigh, MBChBa, Peter Horsewood, PhDa, Gerald J. Gleich, MDb, Gerard Cox, MBa, Frederick E. Hargreave, aMD Journal of Allergy and Clinical Immunology Volume 105, Issue 6, Pages 1162-1168 (June 2000) DOI: 10.1067/mai.2000.106375 Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 1 Flow diagram showing protocol for spiking of sputum with nonradiolabeled IL-5. Journal of Allergy and Clinical Immunology 2000 105, 1162-1168DOI: (10.1067/mai.2000.106375) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 2 Flow diagram showing method for spiking of sputum with radiolabeled IL-5, with and without albumin. Journal of Allergy and Clinical Immunology 2000 105, 1162-1168DOI: (10.1067/mai.2000.106375) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 3 SDS-PAGE electrophoresis of radiolabeled IL-5: IL-5 with no DTT present, final concentration of 47 ng/mL (lane 1) ; sputum processed with DTT and spiked with IL-5, final concentration of 31 ng/mL (lane 2) ; IL-5 treated with DTT, final concentration of 47 ng/mL (lane 3) ; IL-5 without DTT treated with 2-ME, final concentration of 47 ng/mL (lane 4) and 19 ng/mL (lane 5) ; sputum processed with DTT and spiked with IL-5, final concentration of 19 ng/mL (lane 6) and 31 ng/mL (lane 7) . Journal of Allergy and Clinical Immunology 2000 105, 1162-1168DOI: (10.1067/mai.2000.106375) Copyright © 2000 Mosby, Inc. Terms and Conditions