A: Representative hydrophilic interaction liquid chromatography–ultra performance liquid chromatographic profile of N-glycans released from total plasma proteins. A: Representative hydrophilic interaction liquid chromatography–ultra performance liquid chromatographic profile of N-glycans released from total plasma proteins. Glycan peaks (GPs) that exhibited the best discriminative power between HNF1A-MODY and early-onset type 2 diabetes are color coded as follows: GP30 in green, GP38 in yellow, and GP36 in pink. N-Glycan structures contained in the peaks listed above are also depicted as per legend. The levels of GP30 (B), GP36 (C), GP38 (D), and hs-CRP (E) are illustrated according to the type of HNF1A allele variants, alongside ROC curves illustrating the performance of the particular biomarker in differentiating subjects with damaging HNF1A alleles from all other subjects. Subjects are divided into four groups: subjects without the rare HNF1A allele variant (NV), subjects with benign alleles (B), subjects with allele VUS , and subjects with damaging HNF1A alleles (D). Differences in glycan groups and hs-CRP are shown as box plots. Each box represents the 25th to 75th percentile. Lines inside the boxes represent the median. The upper whisker extends from the hinge to the highest value that is within 1.5 × IQR of the hinge, where IQR is the distance between the first and third quartiles. The lower whisker extends from the hinge to the lowest value within 1.5 × IQR of the hinge. Circles indicate outliers. Agata Juszczak et al. Dia Care 2019;42:17-26 ©2019 by American Diabetes Association