WNT1 and WNT7B synergistically upregulate canonical Wnt target genes in YCC11 cells. WNT1 and WNT7B synergistically upregulate canonical Wnt target genes.

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WNT1 and WNT7B synergistically upregulate canonical Wnt target genes in YCC11 cells. WNT1 and WNT7B synergistically upregulate canonical Wnt target genes in YCC11 cells. (A) AXIN2 mRNA abundance in YCC11 cells was assessed by qRT-PCR 24 h after transfection with the indicated combinations of WNT1 and WNT7B expression plasmids. 50 ng of WNT1 in combination with WNT7B is more potent than 100 ng WNT1 or WNT7B alone. The CI was <0.65 for 50 ng, and <0.3 for 100 ng of Wnts, demonstrating strong synergy. Results are mean±s.d. (n=3). P<0.0001 for WNT1 or WNT7B (200 ng) vs WNT1+WNT7B (100 ng each) (ANOVA with Tukey's multiple comparison test). (B–D) The same analysis as in A was performed for the β-catenin target genes LEF1, NKD1 and TCF7. The CI ranged from 0.002–0.21, indicating strong synergy. P<0.0001 for WNT1 or WNT7B (200 ng) vs WNT1+ WNT7B (100 ng each) (ANOVA with Tukey's multiple comparison test). Unt, untransfected; EV, empty vector. Anshula Alok et al. J Cell Sci 2017;130:1532-1544 © 2017. Published by The Company of Biologists Ltd