Apc2 localization and centrosome orientation are altered in Lar mutant GSCs. (A-B′) Apc2 (white, A,B; red, A′,B′) localization in wild-type (A,A′) and.

Slides:

Advertisements

Similar presentations

Volume 7, Issue 3, Pages (May 2014)

Advertisements

Eva Decotto, Allan C. Spradling Developmental Cell

Lacy J. Barton, Belinda S. Pinto, Lori L. Wallrath, Pamela K. Geyer

Dcr-1 Maintains Drosophila Ovarian Stem Cells

Persistent Replicative Stress Alters Polycomb Phenotypes and Tissue Homeostasis in Drosophila melanogaster Severine Landais, Cecilia D’Alterio, D. Leanne.

Volume 6, Issue 4, Pages (April 2010)

Clone patterns and frequencies produced by lines 168 and 688A.

Fluorescence-stained images and respective bright-field images of mouse spleen tissue sections area investigated using Raman spectroscopy. Fluorescence-stained.

Monica Boyle, Chihunt Wong, Michael Rocha, D. Leanne Jones

Volume 18, Issue 4, Pages (April 2010)

Expression of GFP‐Aub transgenes in GSCs, and GSC loss phenotype in armi mutant Expression of GFP‐Aub transgenes in GSCs, and GSC loss phenotype in armi.

Volume 1, Issue 5, Pages (November 2013)

Dedifferentiating Spermatogonia Outcompete Somatic Stem Cells for Niche Occupancy in the Drosophila Testis X. Rebecca Sheng, Crista M. Brawley, Erika.

Decapentaplegic Is Essential for the Maintenance and Division of Germline Stem Cells in the Drosophila Ovary Ting Xie, Allan C Spradling Cell Volume.

Volume 18, Issue 4, Pages (April 2010)

From Stem Cell to Embryo without Centrioles

Boss/Sev Signaling from Germline to Soma Restricts Germline-Stem-Cell-Niche Formation in the Anterior Region of Drosophila Male Gonads Yu Kitadate, Shuji.

Jacob Gonzalez, Hongying Qi, Na Liu, Haifan Lin Cell Reports

Todd Nystul, Allan Spradling Cell Stem Cell

Volume 13, Issue 2, Pages (October 2015)

Volume 19, Issue 18, Pages (September 2009)

Volume 25, Issue 1, Pages (January 2015)

Volume 18, Issue 21, Pages (November 2008)

Zfh-1 Controls Somatic Stem Cell Self-Renewal in the Drosophila Testis and Nonautonomously Influences Germline Stem Cell Self-Renewal Judith L. Leatherman,

Volume 9, Issue 1, Pages (July 2017)

Volume 6, Issue 4, Pages (April 2010)

Cbl protein levels in ago3, twin, and armi mutant GSCs

Intrinsic role of Aub in GSC self‐renewal and differentiation

Let-7-Complex MicroRNAs Regulate the Temporal Identity of Drosophila Mushroom Body Neurons via chinmo Yen-Chi Wu, Ching-Huan Chen, Adam Mercer, Nicholas S.

S. Chodagam, A. Royou, W. Whitfield, R. Karess, J.W. Raff

Volume 10, Issue 1, Pages (January 2006)

Epigenetic Regulation of Stem Cell Maintenance in the Drosophila Testis via the Nucleosome-Remodeling Factor NURF Christopher M. Cherry, Erika L. Matunis

Candidates function in the soma to control GSC and niche cap cell number during larval-pupal transition. Candidates function in the soma to control GSC.

Volume 18, Issue 21, Pages (November 2008)

Drosophila Maelstrom Ensures Proper Germline Stem Cell Lineage Differentiation by Repressing microRNA-7 Jun Wei Pek, Ai Khim Lim, Toshie Kai Developmental.

The POU/Oct Transcription Factor Nubbin Controls the Balance of Intestinal Stem Cell Maintenance and Differentiation by Isoform-Specific Regulation Xiongzhuo.

Physical and genetic interaction between Aub and the CCR4‐NOT complex

Ismail Osman, Jun Wei Pek Stem Cell Reports

Aeri Cho, Masato Kato, Tess Whitwam, Ji Hoon Kim, Denise J. Montell

GSC tumor phenotype in ago3 mutant germaria

Conversion of Quiescent Niche Cells to Somatic Stem Cells Causes Ectopic Niche Formation in the Drosophila Testis Phylis Hétié, Margaret de Cuevas, Erika.

Fig. 3. Hts and Dlg are in a complex at the postsynaptic membrane of larval NMJs.(A–B″) PLA with HtsM and Dlg antibodies (green) performed on third instar.

DHR3 suppresses Phm protein levels.

Rachis extension in drp-1(or1393ts) mutants and GFP::DRP-1 localization. Rachis extension in drp-1(or1393ts) mutants and GFP::DRP-1 localization. (A) The.

Robo3 in the GnRH neuronal system.

Candidates function in the soma during development to control GSC and niche cap cell numbers. Candidates function in the soma during development to control.

Leukocyte infiltration of epidermis and phagocytosis of debris in clint1 mutants. Leukocyte infiltration of epidermis and phagocytosis of debris in clint1.

Expression of FOZI-1 in different M lineage mutants.

F. Christian Bennett, Kieran F. Harvey Current Biology

Apoptotic oocytes in the ovary-like gonadal tissue.

Oocyte polarity is disrupted in the presence of FP41 mutant PFCs

Defective organ development in mab1 mutants.

Postmitotic Prox1-deficient DG cells exhibit pyramidal cell morphology

Apoptotic somatic cells in ovarian connective tissues.

Volume 22, Issue 3, Pages (March 1999)

Two domains of KHC act to position the nucleus.

Exo70 is recruited to the plasma membrane at sites of mechanical wounding. Exo70 is recruited to the plasma membrane at sites of mechanical wounding. NRK.

flr mutations result in defects in the adult eye and larval eye disc.

Lar function is not required for Stat92E accumulation in GSCs

Abnormal adherens junctions between hub cells and GSCs in Lar mutants.

CENP-F staining in prometaphase and metaphase spermatocytes from B6 and Rb/+ mice. CENP-F staining in prometaphase and metaphase spermatocytes from B6.

Epigenetic Regulation of Stem Cell Maintenance in the Drosophila Testis via the Nucleosome-Remodeling Factor NURF Christopher M. Cherry, Erika L. Matunis

Integrin-mediated signaling is required for PFC maturation.

Jan H. Reiling, Kathrin T. Doepfner, Ernst Hafen, Hugo Stocker

Deletion of HtrA1 does not alter vascular or immune cell morphology or distribution in the young-adult mouse neocortex. Deletion of HtrA1 does not alter.

Lack of LC3–GFP clustering in neurons expressing mutant CHMP2B.

Phenotype of S. pombe cells in the presence of B21P2 or LMB

The receptor tyrosine phosphatase Dlar and integrins organize actin filaments in the Drosophila follicular epithelium Jack Bateman, R.Srekantha Reddy,

TDIF-Sensitive Xylem Formation in wox4 Mutants

GATOR1 is required to maintain baseline levels of TORC1 activity in Drosophila. GATOR1 is required to maintain baseline levels of TORC1 activity in Drosophila.

Presentation transcript:

Apc2 localization and centrosome orientation are altered in Lar mutant GSCs. (A-B′) Apc2 (white, A,B; red, A′,B′) localization in wild-type (A,A′) and Lar451/Lar2127 mutant (B,B′) GSCs from 0- to 1-day-old adult Drosophila testes. Apc2 localization and centrosome orientation are altered in Lar mutant GSCs. (A-B′) Apc2 (white, A,B; red, A′,B′) localization in wild-type (A,A′) and Lar451/Lar2127 mutant (B,B′) GSCs from 0- to 1-day-old adult Drosophila testes. Anti-Vasa (green, A′,B′) marks germ cells. (C-D″) Localization of Apc2 (white, C,D; red, C′,D′) in homozygous Lar451 (arrows, C-C″) and Lar2127 (arrows, D-D″) mutant GSC clones 5 days PCI as compared with neighboring heterozygous control GSCs (arrowheads). Homozygous mutant GSCs (green outline, C″,D″) are detected by the absence of anti-GFP immunostaining (green, C′,D′; white C″,D″). Orange solid lines indicate cortical sites in GSCs with prominent Apc2 localization. The star indicates the Hub. (E,F) Quantitation of GSCs with Apc2 localization to the hub-GSC interface versus GSCs with Apc2 localization distributed more broadly over the GSC cortex in (E) GSCs from wild-type versus Lar451/Lar2127 mutant testes and (F) heterozygous and homozygous FRT40A, Lar451 and Lar2127 GSCs. (G,H) Quantitation of GSCs with oriented versus misoriented centrosomes in GSCs with two centrosomes (G) from wild-type versus Lar451/Lar2127 mutant testes and (H) in heterozygous and homozygous FRT40A, Lar451 and Lar2127 GSCs. N, number of GSCs scored for each genotype. P-values of Fisher’s exact test (two-tailed) are shown. Scale bars: 25 μm. Shrividhya Srinivasan et al. Development 2012;139:1381-1390 © 2012.