Monitoring Tissue Regeneration at Single-Cell Resolution Stefano Di Talia, Kenneth D. Poss  Cell Stem Cell  Volume 19, Issue 4, Pages 428-431 (October 2016) DOI: 10.1016/j.stem.2016.09.007 Copyright © 2016 Elsevier Inc. Terms and Conditions

Figure 1 Imaging Approaches to Study Tissue Regeneration (A) Intravital imaging allows live lineage tracing of stem cells in intestinal crypts. Stem cells were permanently labeled with red fluorescent protein expression and tracked for 3 days. Gray lines indicate crypts; white lines indicate clones. Stem cells from either the center (left) or the border (right) of the crypt base contribute new cells to the crypt (adapted from Ritsma et al., 2014 with permission). (B) (Left) Labeling of surface skin cells of zebrafish with ∼70 spectrally distinct colors. (Right) Tracking of hundreds of skin cells during regeneration of an amputated fin (arrows indicate a few reference cells) reveals clear evidence of collective cell migration upon quantification of movements (adapted from Chen et al., 2016 with permission). Cell Stem Cell 2016 19, 428-431DOI: (10.1016/j.stem.2016.09.007) Copyright © 2016 Elsevier Inc. Terms and Conditions