Predicted sizes: Spp = 13.3 kDa Intein + CBT = 34.0 kDa 1 2 3

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Predicted sizes: Spp382 1-121 = 13.3 kDa Intein + CBT = 34.0 kDa 1 2 3 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 1- Total uninduced vector only- Group1 2- Total uninduced spp382– Group1 3- Protein marker 4- Induced Spp382- Group1 5- Eluted control- Group1 6- Eluted Spp382- Group1 7- Bond Sample- Group1 8- Induced Spp382- Group2 9- Eluted control- Group2 10- Eluted Spp382- Group2 11- Bond Sample- Group2 12- Induced Spp382- Group3 13- Eluted control- Group3 14- Eluted Spp382- Group3 15- Bond Sample- Group3 16- Protein marker 17- Induced Spp382- Group4 18- Eluted control- Group4 19- Eluted Spp382- Group4 20- Bond Sample- Group4 21 Induced Spp382- Group5 22- Eluted control- Group5 23- Eluted Spp382- Group5 24- Bond Sample- Group5 25 Induced Spp382- Group6 26- Eluted control- Group6 27- Eluted Spp382- Group6 28- Bond Sample- Group6 29- Protein marker 30 Induced Spp382- Group7 31- Eluted control- Group7 32- Eluted Spp382- Group7 33- Bond Sample- Group7 34 Induced Spp382- Group8 35- Eluted control- Group8 36- Eluted Spp382- Group8 37- Bond Sample- Group8 38- Protein marker Predicted sizes: Spp382 1-121 = 13.3 kDa Intein + CBT = 34.0 kDa