Expression of mRNA erythropoietin in the human retina. Expression of mRNA erythropoietin in the human retina. A: Agarose gel electrophoresis of RT-PCR products from a representative nondiabetic donor. 1, brain (positive control); 2, pancreas (negative control); 3, neuroretina; 4, retinal pigment epithelium; 5, marker 100 bp. B: Erythropoietin (Epo) mRNA measured by real-time RT-PCR (TaqMan Assay) in RPE and neuroretina (R) from nondiabetic and diabetic donors. Kidney (K) was used as positive control. Erythropoietin gene expression data (ΔΔCt) are shown after normalizing with β-actin. Results are presented as the means ± SE. Cristina Hernández et al. Dia Care 2006;29:2028-2033 ©2006 by American Diabetes Association