(A) The result of a qPCR assay comparing fluorescence with cycle number. (A) The result of a qPCR assay comparing fluorescence with cycle number. The results.

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Presentation transcript:

(A) The result of a qPCR assay comparing fluorescence with cycle number. (A) The result of a qPCR assay comparing fluorescence with cycle number. The results for multiple serial dilutions are presented demonstrating the cycle threshold value for each sample. The highest concentration sample (a) crosses the cycle threshold between cycles 20 and 21. The lowest concentration sample (b) crosses the cycle threshold at cycle 41. (B) Schema of 16S rRNA gene demonstrating variable (different between bacterial genera and species) and conserved regions (present in all bacterial rDNA) with placement of primers at conserved regions. After PCR amplification, the variable regions (V5–V7) are then sequenced to identify the bacteria present. (C) Flowchart demonstrating clinical guidance for the use of culture methods, qPCR and broad-range 16S rDNA PCR for sterile site infections where a bacterial cause is suspected. qPCR:quantitative PCR. Amani Patel et al. Arch Dis Child Educ Pract Ed 2017;102:261-264 Copyright © BMJ Publishing Group Ltd & Royal College of Paediatrics and Child Health. All rights reserved.