Generation and genotyping of Syk-AQL mice.

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Generation and genotyping of Syk-AQL mice. Generation and genotyping of Syk-AQL mice. (A) Diagram of targeting vector for the generation of Syk-AQL knock-in mice. (B) Genotype analysis of Syk-AQL knock-in mice showing the migration positions of PCR products generated from Syk and Syk-AQL (AQL) alleles. The mouse genotyped in lane 5 died shortly after birth and lacks the endogenous Syk allele. (C) Genotype analysis of Syk-AQL mice showing migration positions of the PCR products from the BAC transgene (BAC) and the endogenous Syk or introduced Syk-AQL alleles after cleavage with PvuII. Control plasmids containing cDNAs coding for SYK (Syk) or SYK-AQL (AQL) are shown in lanes 1 and 5, respectively. The mouse genotyped in lane 2 contains the Syk-AQL alleles (from both the knocked-in allele and the BAC) and lacks endogenous Syk. (D) Western blot analysis of spleen B cells isolated from wild-type (WT) or from two different Syk-AQL mice (lanes 1 and 2). α-Tubulin (TUB) was detected as a loading control. Wen-Horng Wang et al. ImmunoHorizons 2019;3:254-261 Copyright © 2019 The Authors