Engineering of D1- or D2-MSN-specific YCnano50 or ArchT-EGFP mice.

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Engineering of D1- or D2-MSN-specific YCnano50 or ArchT-EGFP mice. Engineering of D1- or D2-MSN-specific YCnano50 or ArchT-EGFP mice. A, Gene manipulation strategies. Top, tetO cassette was inserted downstream of the Actb polyA signal in tetO knockin mice, but with no gene induction. Middle, tTA-mediated gene induction. tTA binds to the tTA-dependent promoter (tetO) and transactivates the transcription of cDNA in double-transgenic mice. Bottom, The tetO cassette was removed by crossing with Cre mice, yielding Cre recombination. tetO, Tetracycline operator; tTA, tetracycline-controlled transcriptional activator; pA, polyadenylation signal; loxP, locus of X-over P1. B, Strategy for the generation of D2-cDNA (Drd2-tTA::tetO double-transgenic) mice. Drd2-tTA induced the expression of cDNA (YCnano50 or ArchT-EGFP) in D2-MSNs, but not in D1-MSNs. C, Strategy for the generation of D1-cDNA (Pde10a2-tTA::tetO-cDNA; Adora2a-Cre triple-transgenic) mice. Pde10a2-tTA induced the expression of cDNA (YC nano50 or ArchT-EGFP) in D1-MSNs, but not in D2-MSNs by Cre-mediated removal of tetO cassette. D, Left panels, YCnano50 expression in the striatum of adult D2-YC mice. Green represents GFP indirect fluorescence of YCnano50 (left). Blue and red represent D1 and D2 receptors, respectively (middle). Right, Merged image represents that YCnano50 was selectively expressed in D2-MSNs. Right panels, YCnano50 expression in the striatum of adult D1-YC mice. Green represents GFP fluorescence of YCnano50 (left). Red and blue represent D1 and A2a receptor, respectively (middle). Right, Merged image represents that YCnano50 was selectively expressed in D1-MSNs. Akiyo Natsubori et al. J. Neurosci. 2017;37:2723-2733 ©2017 by Society for Neuroscience