Cr-COP1 Activity Is Required for UV-B Acclimation in Chlamydomonas

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Cr-COP1 Activity Is Required for UV-B Acclimation in Chlamydomonas Cr-COP1 Activity Is Required for UV-B Acclimation in Chlamydomonas.(A) Survival of Chlamydomonas UV-B-acclimated and nonacclimated colonies following broadband UV-B stress. Cr-COP1 Activity Is Required for UV-B Acclimation in Chlamydomonas.(A) Survival of Chlamydomonas UV-B-acclimated and nonacclimated colonies following broadband UV-B stress. Top: Representative plates used to quantitate surviving colonies, scanned following 4 d (−UV-B stress treatment) or 8 d growth (+UV-B stress). Bottom: Quantification of surviving colonies. –UV-B, no broadband UV-B stress; +UV-B, 4 h broadband UV-B stress exposure. Means and se are shown (n = 3). Shared letters indicate no statistically significant difference in the means (P > 0.05).(B) Immunoblot analysis of D1 protein level in triplicate cultures of UV-B-acclimated or nonacclimated Chlamydomonas strains before (−UV-B) and following (+UV-B) broadband UV-B stress.(C) UV-B-induced photoinhibition in Chlamydomonas strains as measured by a decrease of the maximum quantum yield of PSII (Fv/Fm) following broadband UV-B stress. Means and se are shown (n = 3). Nonacclimated, grown under weak white light only; Acclimated, grown under equivalent white light supplemented with narrowband UV-B; –UV-B, no broadband UV-B stress; 20/40/60 UV-B, duration of broadband UV-B stress exposure (min). Shared letters indicate no statistically significant difference in the means (P > 0.05).(D) Cr-COP1 activity is required for UV-B-induced expression changes of select Chlamydomonas genes. Individual RT-qPCR analysis of select Chlamydomonas genes. Expression of each gene following 1 h UV-B (+UV-B) is normalized to levels in −UV-B control samples (−UV-B). WT, CC-124 (137c mt-); hit1, Cr-COP1 mutant; hit1/PropsaD:CrCOP1, hit1 complemented by Cr-COP1 expression driven by the psaD promoter. CNRQ, calibrated normalized relative quantities. Kimberley Tilbrook et al. Plant Cell 2016;28:966-983 ©2016 by American Society of Plant Biologists