Delay of tail resorption in trβ crispants during natural metamorphosis

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Fig. 5. Delay of tail resorption in trβ crispants during natural metamorphosis. Delay of tail resorption in trβ crispants during natural metamorphosis. (A) The days from fertilization to stage 61 are indicated in a box plot for uninjected control tadpoles (n=46) and trβ crispants (n=39). There were no significant differences. (B) The days from stage 61 to stage 66 are indicated in a box plot for uninjected control tadpoles (n=45) and trβ crispants (n=38). trβ crispants required significantly more days to reach stage 66 (Welch's t-test, *P<0.05). N values represent the merged total numbers of tadpoles from two independent experiments. Box and whiskers show the interquartile range and maximum and minimum data points, respectively. (C) Time course of tail resorption in uninjected control tadpoles and trβ crispants. Uninjected control tadpoles and trβ crispants of the same age were selected from the same clutch at stage 61 and gill resorption observed at 1 days, 3 days, and 5 days after stage 61 (day 0). Uninjected control tadpoles completed tail resorption by day 5. In contrast, the tail remained in trβ crispants on day 5 (an arrowhead in the high magnification image) and a longer time was required to reach the end of metamorphosis. Yuto Sakane et al. Biology Open 2018;7:bio030338 © 2018. Published by The Company of Biologists Ltd