Galectin-3 Protects Keratinocytes from UVB-Induced Apoptosis by Enhancing AKT Activation and Suppressing ERK Activation Jun Saegusa, Daniel K. Hsu, Wei.

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Galectin-3 Protects Keratinocytes from UVB-Induced Apoptosis by Enhancing AKT Activation and Suppressing ERK Activation Jun Saegusa, Daniel K. Hsu, Wei Liu, Ichiro Kuwabara, Yasuko Kuwabara, Lan Yu, Fu-Tong Liu Journal of Investigative Dermatology Volume 128, Issue 10, Pages 2403-2411 (October 2008) DOI: 10.1038/jid.2008.119 Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Galectin-3 mRNA increases in normal mouse keratinocytes exposed to UVB radiation. Total RNA was extracted from cultured mouse keratinocytes exposed to UVB (100Jm−2) at the indicated time points. Real-time PCR was performed to quantify galectin-3 mRNA. The results were normalized to hypoxanthine ribosyltransferase. Bars represent mean±SD. Similar results were obtained in two additional experiments. Journal of Investigative Dermatology 2008 128, 2403-2411DOI: (10.1038/jid.2008.119) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Gal3−/− keratinocytes are more sensitive to UVB-induced apoptosis in vitro. (a) Flow cytometric analysis of annexin-V-stained keratinocytes. Keratinocytes from gal3+/+ and gal3−/− mice were untreated or irradiated with UVB at the indicated doses in vitro. Cells were harvested 24hours later and apoptosis was measured by flow cytometry with annexin-V-FITC and propidium iodide counterstaining. (b) Analysis of keratinocytes exposed to UVB for pycnotic nuclei. Keratinocytes were plated on coverslips and mock-treated or irradiated with UVB at 100Jm−2, followed by incubation for 24hours. The cells were fixed, stained with Hoechst 33342, and analyzed for nuclear morphology of apoptosis by fluorescence microscopy. Cells with condensed or fragmented nuclei were determined as apoptotic cells. Data are expressed as percentage of apoptotic cells based on counting at least 150 cells. (c) Quantitative detection of histone-associated DNA fragments. Keratinocytes were irradiated with UVB (100Jm−2) or cultured with hydrogen peroxide (25μM) or etoposide (25μM). Twenty-four hours later, histone–DNA complex was detected in an ELISA-based assay. Bars represent mean±SD. *P<0.05. **P<0.001. Similar results were obtained in two additional experiments. Journal of Investigative Dermatology 2008 128, 2403-2411DOI: (10.1038/jid.2008.119) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Higher numbers of apoptotic keratinocytes are induced by UVB irradiation and epicutaneous ovalbumin sensitization in gal3−/− mice. (a and b) Skin sections from gal3+/+ and gal3−/− mice were placed on gauze soaked in RPMI medium and irradiated with UVB ex vivo at the indicated doses. Twenty-four hours after irradiation, samples were processed for TUNEL reaction. (c) Gal3+/+ and gal3−/− mice were treated with three 1-week periods of epicutaneous application of ovalbumin or PBS, each 2 weeks apart. Skin sections were obtained from the treated areas 24hours after the third sensitization period and processed for TUNEL reaction. Apoptotic cells were counted per cm in the skin sections. Bars represent mean±SD. *P<0.05. Journal of Investigative Dermatology 2008 128, 2403-2411DOI: (10.1038/jid.2008.119) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Galectin-3 deficiency does not alter the expression of other galectins. (a) Total RNA was extracted from keratinocytes from gal3+/+ and gal3−/− mice and cDNA was synthesized. Real-time PCR was performed to quantify mRNA. The results were normalized to hypoxanthine ribosyltransferase. (b) Total RNA was extracted from keratinocytes exposed to UVB at the indicated time points. Real-time PCR was performed to quantify galectin-1, -7, and -9 mRNA. The results were normalized to hypoxanthine ribosyltransferase. Bars represent mean±SD. Similar results were obtained in two additional experiments. Journal of Investigative Dermatology 2008 128, 2403-2411DOI: (10.1038/jid.2008.119) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Gal3−/− keratinocytes exhibit higher ERK phosphorylation upon UVB irradiation and ERK inhibitor decreases UVB-induced apoptosis in these cells. (a) Keratinocytes from gal3+/+ and gal3−/− mice were irradiated with UVB (100Jm−2). Cells were collected at indicated times after irradiation, whole cell homogenates were prepared, and total and phosphorylated JNK 1/2, p38, and ERK 1/2 were determined by immunoblotting using specific antibodies. (b) Fold activation was calculated on the basis of phosphorimager analysis. Bars represent mean±SD from three independent experiments. **P<0.01, versus wild type at the same time point. (c) Cells were pre-incubated with 25μM SP600125 (SP) or 50μM PD98059 (PD) for 1hour before exposure to UVB (100Jm−2). Six hours after irradiation, cell lysates were subjected to immunoblotting with each antibody. Twenty-four hours later, histone–DNA complex was detected in an ELISA-based assay. Bars represent mean±SD. *P<0.05. Journal of Investigative Dermatology 2008 128, 2403-2411DOI: (10.1038/jid.2008.119) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 AKT phosphorylation is markedly impaired in gal3−/− keratinocytes. (a) Keratinocytes from gal3+/+ and gal3−/− mice were irradiated with UVB (100Jm−2). Cells were collected at indicated times after irradiation, whole cell homogenates were prepared, and total and phosphorylated AKT levels were determined by immunoblotting using specific antibodies. (b) Fold activation was calculated on the basis of phosphorimager analysis. Bars represent mean±SD from three independent experiments. *P<0.05, **P<0.01, versus wild-type at the same time point. (c) Cells were pre-incubated with 500nM Wortmannin or 20μM LY294002 for 1hour before exposure to UVB (100Jm−2). Six hours after irradiation, cell lysates were subjected to immunoblotting with each antibody. Twenty-four hours later, histone–DNA complex was detected in an ELISA-based assay. Bars represent mean±SD. *P<0.05. Journal of Investigative Dermatology 2008 128, 2403-2411DOI: (10.1038/jid.2008.119) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions