SiRNA-mediated knockdown of class II HDACs abolish the isoproterenol effect on GLUT4 inhibition. 3T3-L1 preadipocytes were transiently transfected with.

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siRNA-mediated knockdown of class II HDACs abolish the isoproterenol effect on GLUT4 inhibition. 3T3-L1 preadipocytes were transiently transfected with scrambled siRNA or HDAC4/5/9-specific siRNA and incubated for 3 days, then transfected a second time with luciferase reporter constructs -895-hGLUT4-luc (for promoter activity) and pRLTK (for transfection efficiency), with and without plasmids encoding GEF and MEF2A, and treated or untreated with forskolin or isoproterenol (+ indicates inclusion in transfection). siRNA-mediated knockdown of class II HDACs abolish the isoproterenol effect on GLUT4 inhibition. 3T3-L1 preadipocytes were transiently transfected with scrambled siRNA or HDAC4/5/9-specific siRNA and incubated for 3 days, then transfected a second time with luciferase reporter constructs -895-hGLUT4-luc (for promoter activity) and pRLTK (for transfection efficiency), with and without plasmids encoding GEF and MEF2A, and treated or untreated with forskolin or isoproterenol (+ indicates inclusion in transfection). Data represent mean and SEM from three independent experiments analyzed by a one-way ANCOVA. #Statistical significance (P < 0.05) vs. the respective scrambled siRNA condition. *P > 0.003 vs. other conditions. Juston C. Weems et al. Diabetes 2012;61:1404-1414 ©2012 by American Diabetes Association