Volume 56, Issue 5, Pages (November 1999)

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Volume 56, Issue 5, Pages 1818-1825 (November 1999) Selective decrease of mRNAs encoding plasma membrane calcium pump isoforms 2 and 3 in rat kidney Ariel J. Caride, Eduardo N. Chini, John T. Penniston, Thomas P. Dousa, M.D., Ph.D. Kidney International Volume 56, Issue 5, Pages 1818-1825 (November 1999) DOI: 10.1046/j.1523-1755.1999.00736.x Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 1 Southern blot analysis of PCR products for plasma membrane Ca2+-ATPase (PMCA)-1 (A), PMCA2 (B), PMCA3 (C), and PMCA4 (D) in rats fed low (LPD)- or high (LPD)-phosphate diet. (E) This shows an agarose gel electrophoresis of the PCR products for β-actin in the same rats. RT-PCR was performed as described in the Methods section. The number of amplification cycles in each case is indicated at the bottom of the figure. Kidney International 1999 56, 1818-1825DOI: (10.1046/j.1523-1755.1999.00736.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 2 Electrophoresis of RT-PCR products for (A) calbindin 28 kDa (band 552 bp) and (B) β-actin in mRNA from kidney cortex of rats fed low-phosphate diet (LPD) or high-phosphate diet (HPD). The procedure is described in the Methods section. The mRNA were pooled from three kidneys for each preparation. Kidney International 1999 56, 1818-1825DOI: (10.1046/j.1523-1755.1999.00736.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 3 Changes in mRNAs encoding the four PMCA isoforms in renal cortex of rats in response to low-phosphate diet (LPD), as compared with high-phosphate diet (HPD). Each bar denotes mean values of LPD/HPD ratios (mean ± SEM). N is the number of observations shown within the bars. The asterisk denotes the values significantly different from zero (*P < 0.01, t-test). Details are discussed in the Methods section. Kidney International 1999 56, 1818-1825DOI: (10.1046/j.1523-1755.1999.00736.x) Copyright © 1999 International Society of Nephrology Terms and Conditions