Microarray analysis of DRG explant cultures of wild-type and ErbB3-deficient mice. Microarray analysis of DRG explant cultures of wild-type and ErbB3-deficient.

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Microarray analysis of DRG explant cultures of wild-type and ErbB3-deficient mice. Microarray analysis of DRG explant cultures of wild-type and ErbB3-deficient mice. (A) DRG explant cultures of wild-type (+/+) and ErbB3-deficient mice (−/−) were isolated from embryos at 12 dpc, cultured for 72 h and imaged under a microscope using phase contrast. Note the absence of migrating Schwann cells in the DRG culture from ErbB3−/− animals. Scale bar: 50 µm. (B) All genes with a higher level of expression in wild-type DRG were grouped into functional categories. (C) Twenty genes with the highest differential expression were grouped into classes. Note the high percentage of ECM molecules (7 out of 20, 35%). (D) Virtual northern blot of mRNA isolated from DRG explant cultures of wild-type (+/+) and ErbB3-deficient mice (−/−). The periostin-specific transcript was detected through hybridisation with a radioactively labelled periostin-specific probe. GAPDH was detected accordingly and was employed as a loading control. Eva Sonnenberg-Riethmacher et al. J Cell Sci 2015;128:3345-3355 © 2015. Published by The Company of Biologists Ltd