Relative expression of clock genes in cultured goldfish liver treated with ghrelin and phospholipase C (PLC) inhibitor. Relative expression of clock genes.

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Relative expression of clock genes in cultured goldfish liver treated with ghrelin, adenylate cyclase (AC) activator and protein kinase A (PKA) inhibitor.

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Relative expression of clock genes in cultured goldfish liver treated with ghrelin and phospholipase C (PLC) inhibitor. Relative expression of clock genes in cultured goldfish liver treated with ghrelin and phospholipase C (PLC) inhibitor. The treatments were carried out for 1 or 5 h. The PLC inhibitor U73122 (1 or 10 µmol l−1) was added 15 min prior to the addition of ghrelin (GRL; 10 nmol l−1). Data obtained by RT-qPCR are shown as means±s.e.m. (n=6, liver aliquots from 6 different fish) in relative units (ΔΔCt method). Asterisks indicate significant inhibition by U73122 of ghrelin stimulation (interaction P<0.05; two-way ANOVA). ‡ indicates a significant stimulation by U73122. Lowercase letters indicate differences among ghrelin and U73122 groups (SNK test). Aída Sánchez-Bretaño et al. J Exp Biol 2017;220:1295-1306 © 2017. Published by The Company of Biologists Ltd