Stimulation of cells with FFAR4 agonist.

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Fig. 5. The measurement of phosphorylation of p53 level in N2A cells under high glucose condition and agmatine treatment. (A, B) p53 phosphorylation levels.

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Stimulation of cells with FFAR4 agonist. Stimulation of cells with FFAR4 agonist. (A) Quantification of lipid droplets from 3D confocal recordings in cells stimulated with the FFAR4 agonist GW9508 (open bars) compared with control cells (closed bars) after oleate (OA) stimulation. The data were normalised to results from fatty-acid-starved cells (starvation) and are expressed as the mean±s.e.m. The P-values are: 15 min, P = 0.8; 180 min, P = 0.0003 (Student's t-test). N.S., non-significant. (B) Western blots to show AKT phosphorylation (pAKT) in the GW9508-stimulated cells. Arndt Rohwedder et al. J Cell Sci 2014;127:3104-3115 © 2014. Published by The Company of Biologists Ltd