Analysis of mutant Best1 protein (Y227N) in testis and sperm function

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Fig. 7. Analysis of mutant Best1 protein (Y227N) in testis and sperm function. Analysis of mutant Best1 protein (Y227N) in testis and sperm function. (A) Percentages of total sperm motility from caudal sperm of CD-1 +/+, +/N and N/N (left graph) (n=6 mice for each genotype; one-way ANOVA, **P<0.01; ***P<0.001) and B6/J (right graph) +/+ and N/N mice (n=4 and six mice, respectively; two-sided paired Student's t-test; n.s., not significant). (B) Phase contrast image of two-stage eggs from a CD-1 +/+ mouse after IVF and quantitative analysis of eggs fertilized with sperm from CD-1 +/+, N/N and −/− males. (C,D) Reproductive capacity of CD-1 females mated with sperm from CD-1 N/N (C) and CD-1 −/− (D) mice and the corresponding +/+ littermates. For each sample, the mean±s.d. is given. Andrea Milenkovic et al. Biology Open 2019;8:bio041335 © 2019. Published by The Company of Biologists Ltd