Allergy to human seminal fluid: Cross-reactivity with dog dander

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Presentation transcript:

Allergy to human seminal fluid: Cross-reactivity with dog dander Maria Basagaña, MD, Borja Bartolomé, MD, PhD, Carlos Pastor, MD, PhD, Ferran Torres, MD, PhD, Rosario Alonso, MD, Fernando Vivanco, MD, PhD, Anna Cisteró-Bahíma, MD, PhD Journal of Allergy and Clinical Immunology Volume 121, Issue 1, Pages 233-239 (January 2008) DOI: 10.1016/j.jaci.2007.10.008 Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 A, SDS-PAGE after Coomassie Brilliant Blue R-250 staining: lane A, HSP; lane M, molecular mass marker. B, IgE immunoblotting: lane P, patient serum; lane C, control serum (pool from nonatopic subject sera). The + and − symbols indicate the presence and absence of β-mercaptoethanol, respectively. Journal of Allergy and Clinical Immunology 2008 121, 233-239DOI: (10.1016/j.jaci.2007.10.008) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 IgE immunoblotting: lanes 1–41, patient sera; lane C, control serum (pool from nonatopic subject sera); lane M, molecular mass markers. Samples are without β-mercaptoethanol. Journal of Allergy and Clinical Immunology 2008 121, 233-239DOI: (10.1016/j.jaci.2007.10.008) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 IgE immunoblotting inhibition: lane C, control serum (pool from nonatopic subject sera); lane 1, patient serum; lane 2, patient serum previously incubated with HSP; lane 3, patient serum previously incubated with dog dander extract; lane 4, patient serum previously incubated with lamb extract; lane M, molecular mass markers. Samples are without β-mercaptoethanol. Journal of Allergy and Clinical Immunology 2008 121, 233-239DOI: (10.1016/j.jaci.2007.10.008) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Identification by means of MS. Peptide fingerprinting of the 28-kd protein is shown. The signaled peaks were analyzed by means of MS/MS, obtaining the amino acid sequence. Journal of Allergy and Clinical Immunology 2008 121, 233-239DOI: (10.1016/j.jaci.2007.10.008) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions