Subsarcolemmal (SS) mitochondrial width (A) and density (B) and intermyofibrillar (IMF) mitochondrial density in close proximity of (D) or distant from (C) lipid droplets, in red muscle of control and ZDF animals. Subsarcolemmal (SS) mitochondrial width (A) and density (B) and intermyofibrillar (IMF) mitochondrial density in close proximity of (D) or distant from (C) lipid droplets, in red muscle of control and ZDF animals. Data are expressed as the means ± SE. Images were taken at 5,800× magnification and the black bar = 5 μm. Width of intermyofibrillar mitochondria was not determined, as this was largely influenced by lipid droplet diameter and not reflective of mitochondrial volume changes. n = 5 animals for each measure. *Significantly different (P < 0.05) from control mitochondria. Graham P. Holloway et al. Diabetes 2010;59:819-828 ©2010 by American Diabetes Association