Alignment of Wt1 genomic regions reveals a highly conserved element upstream of zebrafish wt1a. Alignment of Wt1 genomic regions reveals a highly conserved.

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Alignment of Wt1 genomic regions reveals a highly conserved element upstream of zebrafish wt1a. Alignment of Wt1 genomic regions reveals a highly conserved element upstream of zebrafish wt1a. (A) Genomic sequences upstream of Wt1 from human (Homo sapiens, 8 kb), mouse (Mus musculus, 8 kb), frog (Xenopus tropicalis, 12 kb) and stickleback (Gasterosteus aculeatus, wt1a, 4 kb) were aligned to a 5 kb genomic zebrafish (Danio rerio) sequence upstream of wt1a. The different sequence lengths (4-12 kb) are due to different distances of the conserved region to the first exon (2.1-10.9 kb). The calculation window was 50 bp with a minimum conservation identity of 60%. (B) The conserved regions shown in A from human, mouse, frog, stickleback and zebrafish were aligned at the nucleotide level. For graphical representation the GeneDoc software (www.nrbsc.org/gfx/genedoc/) was used. Nucleotides that are identical in all species are shaded in black and those that are identical in four or three species are shaded in dark and light gray, respectively. The three conserved elements (cons 1-3) are indicated. Frank Bollig et al. Development 2009;136:2883-2892 © 2009. © 2009.