Grg3 is expressed in most β-cells but less frequently in α-cells.

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Grg3 is expressed in most β-cells but less frequently in α-cells. Grg3 is expressed in most β-cells but less frequently in α-cells. A: Triple immunofluorescence of E17.5 pancreas with Grg3 (green), insulin (red), and glucagon (gray) on the same section, showing that most insulin-positive cells are Grg3+ (red arrows), whereas many glucagon-positive cells are Grg3– or have low-level Grg3 expression (gray arrow). B: Immunofluorescence for Grg3, insulin, and glucagon in adult islets, showing that virtually all insulin-positive cells are Grg3+, whereas glucagon-positive cells are Grg3– (white arrows). C: The percentage of Grg3+ β-cells and α-cells was determined at E17.5 (4 animals; 588 α-cells and 932 β-cells analyzed) and adulthood (14 islets from 3 animals; 547 α-cells and 1,479 β-cells analyzed). *P < 0.0001. David E. Metzger et al. Diabetes 2014;63:1804-1816 ©2014 by American Diabetes Association