The MS/MS spectra and MALDI IMS distributions of mannitol and the corresponding polysaccharide containing mannitol are shown. The MS/MS spectra and MALDI IMS distributions of mannitol and the corresponding polysaccharide containing mannitol are shown. The polysaccharide at m/z 345.138 contains an additional hexose residue, and the polysaccharide at m/z 689.272 contains two additional hexose sugar residues. The molecular network corresponding to the polysaccharide family is shown in Fig. S6B in the supplemental material. Neha Garg et al. mSystems 2016; doi:10.1128/mSystems.00139-16