Unaltered presynaptic transmitter secretion during pharmacological treatments. Unaltered presynaptic transmitter secretion during pharmacological treatments.

Slides:

Advertisements

Similar presentations

C. The giant presynaptic terminals of bipolar neurons in the retina are more than 5 μm in diameter, permitting direct patch-clamp recordings of membrane.

Advertisements

Volume 28, Issue 1, Pages (October 2000)

Density of dendritic spines on L2/3 pyramidal neurons is greater in visual cortex of germline PirB−/− mice than in PirB+/+ mice at P30. Density of dendritic.

Volume 56, Issue 6, Pages (December 2007)

Visualization of Synaptic Activity in Hippocampal Slices with FM1-43 Enabled by Fluorescence Quenching Jason L Pyle, Ege T Kavalali, Sukwoo Choi, Richard.

Volume 58, Issue 1, Pages (April 2008)

Coincident Pre- and Postsynaptic Activity Modifies GABAergic Synapses by Postsynaptic Changes in Cl− Transporter Activity Melanie A Woodin, Karunesh.

Bidirectional Modification of Presynaptic Neuronal Excitability Accompanying Spike Timing-Dependent Synaptic Plasticity Cheng-yu Li, Jiang-teng Lu, Chien-ping.

David Zenisek, Gary Matthews Neuron

Kinetics of Releasable Synaptic Vesicles and Their Plastic Changes at Hippocampal Mossy Fiber Synapses Mitsuharu Midorikawa, Takeshi Sakaba Neuron

The Reduced Release Probability of Releasable Vesicles during Recovery from Short- Term Synaptic Depression Ling-Gang Wu, J.Gerard G Borst Neuron Volume.

Endogenous phosphorylation of Atp1a3 is regulated by phosphatases.

Recruitment of N-Type Ca2+ Channels during LTP Enhances Low Release Efficacy of Hippocampal CA1 Perforant Path Synapses Mohsin S. Ahmed, Steven A. Siegelbaum

Volume 21, Issue 4, Pages (October 1998)

Volume 87, Issue 6, Pages (December 1996)

Volume 37, Issue 2, Pages (January 2003)

Volume 68, Issue 6, Pages (December 2010)

A Genetic Analysis of Synaptic Development

CAPS-1 and CAPS-2 Are Essential Synaptic Vesicle Priming Proteins

Bo Li, Ran-Sook Woo, Lin Mei, Roberto Malinow Neuron

Tiago Branco, Kevin Staras, Kevin J. Darcy, Yukiko Goda Neuron

Marta Navarrete, Alfonso Araque Neuron

EPS15L1 has a nonredundant role in neurons.

Endogenous Cannabinoids Mediate Retrograde Signals from Depolarized Postsynaptic Neurons to Presynaptic Terminals Takako Ohno-Shosaku, Takashi Maejima,

The Use of pHluorins for Optical Measurements of Presynaptic Activity

Volume 57, Issue 3, Pages (February 2008)

Hui Jiang, Wei Guo, Xinhua Liang, Yi Rao Cell

Volume 21, Issue 4, Pages (October 1998)

Suzanne Paradis, Sean T Sweeney, Graeme W Davis Neuron

Depolarization-induced postsynaptic NT secretion depends on Ca2+ influx. Depolarization-induced postsynaptic NT secretion depends on Ca2+ influx. Hippocampal.

Propagation is independent of synaptic transmission.

Activation of the MAPK/Erk cascade is involved in the formation of functional synapses between B2 neurons. Activation of the MAPK/Erk cascade is involved.

Synaptic density is significantly impaired in male FBN-ARO-KO mice.

Macaque Satb2-RGCs are synaptically connected to geniculate neurons.

ATF4 overexpression preserves parkin protein levels after treatment with dopaminergic neuronal toxins. ATF4 overexpression preserves parkin protein levels.

Neuronal and glial expression of Nogo-A and synaptic localization of Nogo-A and NgR 1 in the motor cortex. Neuronal and glial expression of Nogo-A and.

The effect of benzolamide on NH4+-induced pHi transients in cultured mouse hippocampal neurons. The effect of benzolamide on NH4+-induced pHi transients.

Ethanol specifically inhibits presynaptic vesicle release at excitatory synapses. Ethanol specifically inhibits presynaptic vesicle release at excitatory.

MSK1 is necessary for BDNF-dependent CREB phosphorylation in area CA1.

HIF-1 is required for IGF-1-induced VEGF mRNA expression and protein secretion. HIF-1 is required for IGF-1-induced VEGF mRNA expression and protein secretion.

Physiological characteristics of large fastigial glycinergic neurons resemble those of large glutamatergic neurons, not small glycinergic neurons. a, Action.

Purkinje cells inhibit large fastigial glycinergic neurons

NCS-Rapgef2-dependent ERK activation in mouse NAc following D1 dopamine receptor agonist SKF and psychostimulants D-amphetamine or cocaine administration.

Volume 57, Issue 3, Pages (February 2008)

PDL-coated beads induce the formation of functional presynaptic terminals within 24 h. PDL-coated beads induce the formation of functional presynaptic.

BACHD mice demonstrate decreases in synaptic activity of medium-sized spiny neurons at 6 months. BACHD mice demonstrate decreases in synaptic activity.

Depolarization-evoked firing activity of hippocampal bipolar neurons that have been dissociated from F344/NSlc and Hiss rats. Depolarization-evoked firing.

A, B, Startle magnitude and PPI after treatment with CNO

Knock-out of NSG2 decreases mEPSC frequency.

AP-induced axonal swelling and subsequent migration of microglial processes to the periaxonal area. AP-induced axonal swelling and subsequent migration.

Exposure of in vitro sensorimotor synaptic connections to RNA from trained animals enhanced the strength of a subpopulation of synapses. Exposure of in.

Electron micrograph of large IC neuron with dense VGLUT2+ axosomatic endings. a, VGLUT2+ terminals contain an electron-dense reaction product and surround.

Arc/Arg3.1 Mediates Homeostatic Synaptic Scaling of AMPA Receptors

SSC LTP in narrow-field vertical neurons is induced by SO stimulation at 20 Hz for 20 s. sSC LTP in narrow-field vertical neurons is induced by SO stimulation.

OGD treatment increased tonic current through an oxidative reaction.

Effects of receptor antagonists, Ca2+ channel blocker, Ca2+depletion, and protein kinase inhibitors on CREB phosphorylation in cultured hippocampal neurons.

Rate of current rundown through VDCC and NMDA receptor channels is reduced in gelsolin-deficient hippocampal neurons. Rate of current rundown through VDCC.

Proximal E-box sequence in Bdnf promoter 4 functions as a transcriptional suppressor. Proximal E-box sequence in Bdnf promoter 4 functions as a transcriptional.

Target-specific DSI at TRN synapses.

Venkatesh N Murthy, Thomas Schikorski, Charles F Stevens, Yongling Zhu

MSCaTs measured by GCaMP6f imaging reflect NMDAR activation at individual synapses following spontaneous single vesicle release. mSCaTs measured by GCaMP6f.

At P23, dendritic spine density on L2/3 pyramidal cells in PirB−/− visual cortex is similar to that of PirB+/+. At P23, dendritic spine density on L2/3.

A, Representative examples of Ca currents elicited by repetitive 3 Hz depolarizing voltage steps to 0 mV from a holding potential of −40 mV from a CA1.

A, Representative voltage-clamp recordings from a CRF1+ neuron during superfusion of GBZ (100 μm), followed by PTX (100 μm, top trace) and during superfusion.

Gene expression in the PFC of mice with an inducible neuron-specific bdnf knock-out in embryogenesis. Gene expression in the PFC of mice with an inducible.

Increased spike ADP in pilocarpine-treated rats is sensitive to spermine. Increased spike ADP in pilocarpine-treated rats is sensitive to spermine. A,

Restricted neurite outgrowth in explant cultures of Nmnat2gtE/gtE DRGs and SCGs at different embryonic stages. Restricted neurite outgrowth in explant.

Partial restoration of impaired LTP in hippocampal slices from 12-month-old NCAM−/− mice through inhibition of GluN2B-containing receptor signaling. Partial.

The effect of roscovitine on Ca2+-dependent glutamate release from synaptosomes prepared from rat brains in response to membrane depolarization with 1.

Ca2+ Influx through Distinct Routes Controls Exocytosis and Endocytosis at Drosophila Presynaptic Terminals Hiroshi Kuromi, Atsuko Honda, Yoshiaki Kidokoro

Presentation transcript:

Unaltered presynaptic transmitter secretion during pharmacological treatments. Unaltered presynaptic transmitter secretion during pharmacological treatments. A , Presynaptic terminals of hippocampal neurons were loaded with FM 4-64 at 10 DIV. Presynaptic secretion of FM 4-64 was elicited with elevated K+ (50 mm; 40 s). B , Boxed area in A shown at higher magnification. Pictures show FM 4-64 fluorescence in presynaptic terminals at indicated time points (0 s, start of depolarization; compare C ). C , Average time course of FM release in untreated (control) and treated neurons (50 μm nifedipine). Note the similar time courses of FM secretion, indicating unaltered presynaptic secretion after the drug treatment also used in NT secretion experiments. D , Average residual FM 4-64 fluorescence after 40 s depolarization for different treatments as indicated. All fluorescence values are given relative to the fluorescence levels at the start of the depolarization. Note that the different drug treatments did not significantly change presynaptic release of transmitters, indicating intact synaptic transmitter release under our recording conditions. neg. control, Negative control; rel., relative. Richard Kolarow et al. J. Neurosci. 2007;27:10350-10364 ©2007 by Society for Neuroscience