PGC-1α activation is required for autophagy flux initiation in ARPE-19 cells. PGC-1α activation is required for autophagy flux initiation in ARPE-19 cells.

Slides:

Advertisements

Similar presentations

SIRT3 Protects Rotenone-induced Injury in SH-SY5Y Cells by Promoting Autophagy through the LKB1-AMPK-mTOR Pathway Zhang Meng 1 ;Deng Yong-Ning 1 ;Zhang.

Advertisements

Figure 1. Intact autophagosome–lysosome fusion, but impaired autolysosome clearance in NPC1-deficient cells. (A) Representative images of human dermal.

AntimiR-30b Inhibits TNF-α Mediated Apoptosis and Attenuated Cartilage Degradation through Enhancing Autophagy Cell Physiol Biochem 2016;40:

The ER-Mitochondria Tethering Complex VAPB-PTPIP51 Regulates Autophagy

Genetic alterations in the PI3K–PTEN–AKT pathway detected during disease progression and their functional impacts. Genetic alterations in the PI3K–PTEN–AKT.

Mitochondrial ER Contacts Are Crucial for Mitophagy in Yeast

Volume 25, Issue 13, Pages (June 2015)

ZKSCAN3 Is a Master Transcriptional Repressor of Autophagy

CHK1 downregulation upon ERG overexpression.

Disrupted dynamics of early stages of differentiation perturbs neural cell fate specification. Disrupted dynamics of early stages of differentiation perturbs.

Schematic representation of the autophagy pathway

Volume 6, Issue 5, Pages (May 2016)

LATS2 promotes death of lumB cells.

Volume 3, Issue 1, Pages (January 2013)

Fig. 2 Fas controls IL-1RA–sEV secretion in murine MSCs.

Propionibacterium acnes Induces Autophagy in Keratinocytes: Involvement of Multiple Mechanisms Klára Megyeri, László Orosz, Szilvia Bolla, Lilla Erdei,

STARD3 favors cholesterol accumulation in endosomes

VAP protein knockdown abolishes STARD3‐mediated cholesterol accumulation in endosomes VAP protein knockdown abolishes STARD3‐mediated cholesterol accumulation.

Volume 68, Issue 4, Pages e6 (November 2017)

BDNF expression in the cerebellum and brain stem region.

STARD3‐mediated cholesterol accumulation in endosomes occurs at the expense of plasma membrane STARD3‐mediated cholesterol accumulation in endosomes occurs.

Gloria Slattum, Yapeng Gu, Roger Sabbadini, Jody Rosenblatt

BMDC-EV attract immune cells in lymph nodes similarly as in the skin.

VAP protein knockdown abolishes STARD3‐mediated cholesterol accumulation in endosomes VAP protein knockdown abolishes STARD3‐mediated cholesterol accumulation.

LXRα enhances glucose uptake and O‐GlcNAc signaling via activation of the hexosamine biosynthetic pathway (HBP) in cultured cardiomyocytes LXRα enhances.

MAPK14 regulates ROS generation in GS cells.

Immunolocalization and quantification of mitochondrial and EMT markers in PGC-1–floxed mice 4 mo after subretinal AAV injection. Immunolocalization and.

OCT and ERG analysis of wild-type mice 2 and 4 wk after injection with AAVs. OCT and ERG analysis of wild-type mice 2 and 4 wk after injection with AAVs.

Morphologic and functional evaluation of adult conditional PGC-1 knockout mice. Morphologic and functional evaluation of adult conditional PGC-1 knockout.

Morphological and functional evaluation of adult PGC-1–floxed mice 2 and 4 wk after subretinal AAV injection. Morphological and functional evaluation of.

FGF/ERK inhibition accelerates neural fate conversion of EpiSCs.

Expression of tomoregulin-1 in wild-type and cardiac hypertrophy mouse myocardium. Expression of tomoregulin-1 in wild-type and cardiac hypertrophy mouse.

Fig. 5. Annexin A11 mutations disrupt binding to calcyclin.

Effect of Z-FA-FMK on the expression of SMN proteins in SMA patient iPSCs and iPSC-derived motor neurons. Effect of Z-FA-FMK on the expression of SMN proteins.

Identification of Z-FA-FMK as a potent compound that increases SMN protein expression in HEK293 and patient fibroblast cells. Identification of Z-FA-FMK.

Chd5 deficiency leads to compromised expression of the repressive histone mark H3K27me3 and up-regulation of ribosomal protein genes. Chd5 deficiency leads.

Effect of PGC-1α silencing on RPE mitochondrial replication genes and glycolytic function. Effect of PGC-1α silencing on RPE mitochondrial replication.

RPE dedifferentiation and mesenchymal transition after PGC-1 deletion.

Validation of GFP and BMP-2 expression in lentivirus-transduced neonatal human skin fibroblasts. Validation of GFP and BMP-2 expression in lentivirus-transduced.

ONC201 activates the ISR. ONC201 activates the ISR. (A) Western blotting analysis for ATF4, CHOP, ATF3, and TRB3 on lysates from HCT116 cells cultured.

Dissociation of HP1α from H3K9me3 is critical to potentiation of steady-state ATM activity. Dissociation of HP1α from H3K9me3 is critical to potentiation.

Sustained PGC-1α loss of function triggers mesenchymal transition.

AAS-dependent induction of autophagic flux in ARPE-19.

KIF13A depletion leads to a drop in virus titers, without affecting viral protein expression. KIF13A depletion leadsto a drop in virus titers, without.

Impaired in vitro adipogenic differentiation parallels elevated HDAC9 expression. Impaired in vitro adipogenic differentiation parallels elevated HDAC9.

Fig. 2. Acetylation stiffens primary cilia.

Hepatocyte Growth Factor Regulates the miR-206-HDAC4 Cascade to Control Neurogenic Muscle Atrophy following Surgical Denervation in Mice Wooshik Choi,

PGC-1α silencing in RPE cells promotes mitochondrial dysfunction and oxidative stress. PGC-1α silencing in RPE cells promotes mitochondrial dysfunction.

Association of NM-HA and NM-GFP with SGs is transient.

Volume 17, Issue 1, Pages (July 2015)

ATF4 overexpression preserves parkin protein levels after treatment with dopaminergic neuronal toxins. ATF4 overexpression preserves parkin protein levels.

Aβ-mediated Ras-MAPK signaling and Cyclin D1 expression in B103 cells are dependent on APP expression and can be reversed with MEK or Ras inhibition. Aβ-mediated.

A New Long Noncoding RNA ALB Regulates Autophagy by Enhancing the Transformation of LC3BI to LC3BII during Human Lens Development Qiuli Fu, Zhenwei Qin,

Overexpression of IL-27 upregulates expression of multiple IR by T cells in vivo. Overexpression of IL-27 upregulates expression of multiple IR by T cells.

CRISPRa induction of Bdnf mRNA increases spike and burst frequency in hippocampal neurons cultured on microelectrode arrays (MEAs). CRISPRa induction of.

Activity regulates eEF2K-dependent spine morphogenesis.

BME treatment increases granzyme B expression in NK 3.3 cells.

Volume 26, Issue 15, Pages (August 2016)

Microarray analysis of global gene expression profiles of PC-3 and derivative cell lines. Microarray analysis of global gene expression profiles of PC-3.

DNA demethylation reactivates GSN expression in gastric cancer cells.

Autophagy Activation Is Mediated by the Secretion of T3Es.

Microfluidic platform for cell phenotype and gene function analysis

Osteoactivin is overexpressed in aggressively bone metastatic 4T1 subpopulations versus weakly bone metastatic breast cancer populations. Osteoactivin.

Fig. 5 C9orf72 knockdown disrupts autophagy induction.

NRAS-mediated resistance to vemurafenib (Vem) is associated with restored glycolysis and can be overcome by combination with a glycolysis inhibitor. NRAS-mediated.

Volume 23, Issue 10, Pages (June 2018)

Interaction of NHEJ and HR pathways in PCO cultures.

Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells. Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells.

IL-17 signaling upregulates ABIN-1 mRNA but downregulates protein.

TAMs upregulate DNMT1 in gastric cancer cells through the CCL5/CCR5/STAT3 pathway. TAMs upregulate DNMT1 in gastric cancer cells through the CCL5/CCR5/STAT3.

Presentation transcript:

PGC-1α activation is required for autophagy flux initiation in ARPE-19 cells. PGC-1α activation is required for autophagy flux initiation in ARPE-19 cells. (A) Immunodetection of late-stage endosomes (LAMP-1, red) in shPGC1A and shCtrl ARPE-19 matured for up to 21 d. Arrows indicate dilated late endosomes in shPGC1A cells. (B) Expression profiles of autophagy-related genes MAP1LC3B and WIPI (initiation of autophagosomes), ATG4D (autophagosome maturation), and ATG9B (lysosomal degradation pathway) (n = 3–4, per condition and time points). Scale bar is 10 μm. (C) Changes in autophagosome biogenesis-related genes ATG4D, WIPI1, MAP1LC3B, and MCOLN1 in parental ARPE-19 cells 24 h after adenoviral-mediated PGC-1α overexpression (n = 3). (D) PGC-1α and autophagy-related genes LAMP1, MAP1LC3B, ATG4D, and WIPI1 expression quantified by qPCR in parental ARPE-19 cells after AAS for 24 h. Untreated (Unt) cells were used as controls (n = 4). (E–G) Western blot analysis for LC3B (E); polyubiquitin-binding p62 protein sequestosome (p62) (F); phospho (Thr172) and total AMPKα (G) in shCtrl and shPGC1A cells 2 h after AAS. Immunoblots are representative of n = 3–4 experiments per conditions. (H) APOE protein expression in shCtrl and shPGC1A cells ARPE-19 cells after 14 d of maturation (n = 4). For all blots, GAPDH was used as the loading control. Error bars are means ± SEM. Data were analyzed by multiple unpaired t test comparisons using the Holm-Sidak method (B–D, H) or ANOVA followed by Tukey’s multiple comparison test (E–G). *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001 versus respective controls. #P ≤ 0.05, ##P ≤ 0.01 and ###P ≤ 0.001 = shPGC1A versus shCtrl under AAS. Mariana Aparecida Brunini Rosales et al. LSA 2019;2:e201800212 © 2019 Rosales et al.