Application of 2-DG, a competitive inhibitor of glycolysis, has a minor effect on mitochondrial transport. Application of 2-DG, a competitive inhibitor.

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Application of 2-DG, a competitive inhibitor of glycolysis, has a minor effect on mitochondrial transport. Application of 2-DG, a competitive inhibitor of glycolysis, has a minor effect on mitochondrial transport. (A) Kymograph of distribution and movements of Mitotracker-labeled mitochondria. The position and time of drug application (200 mM 2-DG, and Lucifer Yellow in DMEM) is shown in blue. (B) Hand-drawn traces of anterograde (blue) and retrograde (red) movements used to quantify the mitochondrial transport. (C-F) Normalized anterograde and retrograde transport. Error bars are the normalized 95% confidence intervals (n=5 experiments). (D) Decreases in anterograde and retrograde transport start but do not reach significant levels. (E) After 5 minutes of drug application both anterograde and retrograde transport are modestly (P<0.05) decreased for each direction of transport past the point of drug application. (F) Anterograde and retrograde transport after drug application is stopped. Kyle E. Miller, and Michael P. Sheetz J Cell Sci 2004;117:2791-2804 © The Company of Biologists Limited 2004