Phosphoribosomes for Fingerprinting Neurons

Slides:

Advertisements

Similar presentations

Structure of a Ubiquitin E1-E2 Complex: Insights to E1-E2 Thioester Transfer Shaun K. Olsen, Christopher D. Lima Molecular Cell Volume 49, Issue 5, Pages.

Advertisements

SOD1 Integrates Signals from Oxygen and Glucose to Repress Respiration Amit R. Reddi, Valeria C. Culotta Cell Volume 152, Issue 1, Pages (January.

Individualized Medicine from Prewomb to Tomb Eric J. Topol Cell Volume 157, Issue 1, Pages (March 2014) DOI: /j.cell Copyright.

The RAG Recombinase Dictates Functional Heterogeneity and Cellular Fitness in Natural Killer Cells Jenny M. Karo, David G. Schatz, Joseph C. Sun Cell Volume.

The Cell-Non-Autonomous Nature of Electron Transport Chain-Mediated Longevity Jenni Durieux, Suzanne Wolff, Andrew Dillin Cell Volume 144, Issue 1, Pages.

Bleach Activates a Redox-Regulated Chaperone by Oxidative Protein Unfolding J. Winter, M. Ilbert, P.C.F. Graf, D. Özcelik, U. Jakob Cell Volume 135, Issue.

What We Talk About When We Talk About Fat Evan D. Rosen, Bruce M. Spiegelman Cell Volume 156, Issue 1, Pages (January 2014) DOI: /j.cell

Molecular and Genetic Properties of Tumors Associated with Local Immune Cytolytic Activity Michael S. Rooney, Sachet A. Shukla, Catherine J. Wu, Gad Getz,

Transcriptional Control of Gene Expression by MicroRNAs Basel Khraiwesh, M. Asif Arif, Gotelinde I. Seumel, Stephan Ossowski, Detlef Weigel, Ralf Reski,

Common Sense about Taste: From Mammals to Insects David A. Yarmolinsky, Charles S. Zuker, Nicholas J.P. Ryba Cell Volume 139, Issue 2, Pages (October.

The Pathobiology of Vascular Dementia Costantino Iadecola Neuron Volume 80, Issue 4, Pages (November 2013) DOI: /j.neuron Copyright.

UvrD Helicase Unwinds DNA One Base Pair at a Time by a Two-Part Power Stroke Jae Young Lee, Wei Yang Cell Volume 127, Issue 7, Pages (December.

Nucleation and Transport Organize Microtubules in Metaphase Spindles Jan Brugués, Valeria Nuzzo, Eric Mazur, Daniel J. Needleman Cell Volume 149, Issue.

Eph-Ephrin Bidirectional Signaling in Physiology and Disease Elena B. Pasquale Cell Volume 133, Issue 1, Pages (April 2008) DOI: /j.cell

Xenobiotics Shape the Physiology and Gene Expression of the Active Human Gut Microbiome Corinne Ferrier Maurice, Henry Joseph Haiser, Peter James Turnbaugh.

Cancer Metabolism Cell Volume 148, Issue 3, (February 2012) DOI: /j.cell Copyright © 2012 Terms and Conditions Terms and Conditions.

The Monarch Butterfly Genome Yields Insights into Long-Distance Migration Shuai Zhan, Christine Merlin, Jeffrey L. Boore, Steven M. Reppert Cell Volume.

Dynein Anchors Its mRNA Cargo after Apical Transport in the Drosophila Blastoderm Embryo Renald Delanoue, Ilan Davis Cell Volume 122, Issue 1, Pages

Encoding of Both Analog- and Digital-like Behavioral Outputs by One C. elegans Interneuron Zhaoyu Li, Jie Liu, Maohua Zheng, X.Z. Shawn Xu Cell Volume.

Neuronal Cell Types and Connectivity: Lessons from the Retina H. Sebastian Seung, Uygar Sümbül Neuron Volume 83, Issue 6, Pages (September 2014)

Nuclear Receptors, RXR, and the Big Bang Ronald M. Evans, David J. Mangelsdorf Cell Volume 157, Issue 1, Pages (March 2014) DOI: /j.cell

Writing Memories with Light-Addressable Reinforcement Circuitry Adam Claridge-Chang, Robert D. Roorda, Eleftheria Vrontou, Lucas Sjulson, Haiyan Li, Jay.

The Landscape of Microsatellite Instability in Colorectal and Endometrial Cancer Genomes Tae-Min Kim, Peter W. Laird, Peter J. Park Cell Volume 155, Issue.

The Good Fat Cell Volume 147, Issue 7, (December 2011) DOI: /j.cell Copyright © 2011 Terms and Conditions Terms and Conditions.

Molecular Motors in Neurons: Transport Mechanisms and Roles in Brain Function, Development, and Disease Nobutaka Hirokawa, Shinsuke Niwa, Yosuke Tanaka.

WHAMM Is an Arp2/3 Complex Activator That Binds Microtubules and Functions in ER to Golgi Transport Kenneth G. Campellone, Neil J. Webb, Elizabeth A. Znameroski,

The Mitochondrial Basis of Aging Nuo Sun, Richard J. Youle, Toren Finkel Molecular Cell Volume 61, Issue 5, Pages (March 2016) DOI: /j.molcel

Quantitative Single-Cell Approaches to Stem Cell Research Martin Etzrodt, Max Endele, Timm Schroeder Cell Stem Cell Volume 15, Issue 5, Pages (November.

Metabolism by Remote Control

EJCs at the Heart of Translational Control

Unlocking CNS Cell Type Heterogeneity

RNase E Finds Some sRNAs Stimulating

The Tail End of Membrane Insertion

A Sympathetic View on Fat by Leptin

Wing Y. Chang, William L. Stanford Cell Stem Cell

Should I Stay or Should I Go: Neuromodulators of Behavioral States

Reconstructing Complex Tissues from Single-Cell Analyses

In This Issue Cell Volume 158, Issue 5, (August 2014)

Volume 131, Issue 1, Pages (October 2007)

A New Handle for a Hot Topic: Genetic Markers for Warm-Sensing

A Dead End for MicroRNAs

Joseph W. Briggs, Jonathan D. Dinman Molecular Cell

Eukaryotic Transcription Activation: Right on Target

Synaptic Plasticity of Feeding Circuits: Hormones and Hysteresis

Joseph W. Briggs, Jonathan D. Dinman Molecular Cell

Volume 152, Issue 1, (January 2013)

Systems Biology Strikes Gold

Volume 130, Issue 6, (September 2007)

Modeling Transcriptome Dynamics in a Complex World

Proteins Kinases: Chromatin-Associated Enzymes?

Modifications on Translation Initiation

Volume 143, Issue 6, (December 2010)

Brandon J. Thomas, Stephen T. Smale Immunity

Volume 166, Issue 4, Pages (August 2016)

Functional Enhancer Screening in Single Cells

A FIRE-y PAGE in the Computational Analysis of Cancer Profiles

The Plasticity of Aging: Insights from Long-Lived Mutants

Bernard Mulvey, Joseph D. Dougherty Cell

Learning from the Uncontrollable

Finding the Roots of Adult Neurogenesis

Volume 163, Issue 4, (November 2015)

Volume 163, Issue 2, (October 2015)

The Frustrated Gene: Origins of Eukaryotic Gene Expression

Volume 25, Issue 2, Pages (January 2007)

Flies Sleep on It, or Fuhgeddaboudit!

Volume 134, Issue 6, (September 2008)

David Hubel and Torsten Wiesel

In This Issue Cell Volume 145, Issue 3, (April 2011)

Volume 148, Issue 1, (January 2012)

Targeting Protein Stability with a Small Molecule

Presentation transcript:

Phosphoribosomes for Fingerprinting Neurons Marcelo O. Dietrich, Tamas L. Horvath Cell Volume 151, Issue 5, Pages 934-936 (November 2012) DOI: 10.1016/j.cell.2012.11.006 Copyright © 2012 Elsevier Inc. Terms and Conditions

Figure 1 Representation of the Phosphorylated Ribosome Capture Technique Silent neurons have decreased ribosome activity, which is represented by low levels of S6 phosphorylation. When neurons are activated via specific stimuli, S6 becomes phosphorylated, and immunoprecipitation of the ribosome complex with pS6 antibodies yields mRNA synthesized during activation. By combining behavioral tasks or other tools to manipulate cell activity, it is possible to pull down ribosomes from target areas in the brain to identify the molecular profile of activated cells under the given circumstance. After validation of genes identified during molecular profiling, animal models can be generated to further manipulate and study the circuitry. Subsequently, techniques to activate or deactivate neurons can be used to test the role of a specific subset of cells in behavior and other regulatory systems. It is also possible to combine these techniques with tracing technologies to investigate projection fields. Cell 2012 151, 934-936DOI: (10.1016/j.cell.2012.11.006) Copyright © 2012 Elsevier Inc. Terms and Conditions