Islet morphology at normoxic or hypoxic culture conditions.

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Islet morphology at normoxic or hypoxic culture conditions. Islet morphology at normoxic or hypoxic culture conditions. Isolated human islets, stained with hematoxylin and eosin. Islets were cultivated at normoxic conditions for 24 h (A) and 48 h (B) and at hypoxic conditions for 24 h (C, E, and F) and 48 h (D). An enlarged representative section for healthy and apoptotic nuclei and necrotic areas is shown in (F). Arrowhead, healthy nuclei; arrow, condensed, pycnotic nuclei. Islets cultivated under normoxic conditions (A and B) show less pycnotic dark nuclei and have a more intact structure then islets under hypoxic conditions (C–F), which show apoptotic/necrotic cores, of which the majority is preferentially found in the core of large islets. Roger Lehmann et al. Diabetes 2007;56:594-603 ©2007 by American Diabetes Association