S Evsikov, Ph.D., J Cieslak, Y Verlinsky, Ph.D.

Slides:

Advertisements

Similar presentations

Pere Colls, Ph. D. , Tomas Escudero, M. Sc. , Natalie Cekleniak, M. D

Advertisements

Tracy I. George, Joanna E. Wrede, Charles D. Bangs, Athena M

Loss of Function of MULTICOPY SUPPRESSOR OF IRA 1 Produces Nonviable Parthenogenetic Embryos in Arabidopsis Anne-Elisabeth Guitton, Frédéric Berger

Genome Transfer Prevents Fragmentation and Restores Developmental Potential of Developmentally Compromised Postovulatory Aged Mouse Oocytes Mitsutoshi.

Anil K Dubey, Ph. D. , Jose R Cruz, M. D. , Beth Hartog, M. D

Increased fetal abortion rate in autoimmune thyroid disease is related to circulating TPO autoantibodies in an autoimmune thyroiditis animal model Yin.

Conversion and non-conversion approach to preimplantation diagnosis for chromosomal rearrangements in 475 cycles Anver Kuliev, Jeanine Cieslak Janzen,

Composition of single-step media used for human embryo culture

Blastocyst transfer does not cause a sex-ratio imbalance

Successful delivery after the transfer of embryos obtained from a cohort of incompletely in vivo matured oocytes at retrieval time Patricia Fauque, M.D.,

Abnormally fertilized oocytes can result in healthy live births: improved genetic technologies for preimplantation genetic testing can be used to rescue.

Mahmut Balkan, Ph. D. , Hatun Duran, M. D. , Abdurrahman Önen, M. D

Recombinant human leukemia inhibitory factor enhances the development of preimplantation mouse embryo in vitro Horng-Der Tsai, M.D., Chi-Chen Chang,

Huei-Wen Chen, Ph.D., Wen-Sheng Jiang, M.S., Chii-Ruey Tzeng, M.D.

Potential use of repeated fluorescence in situ hybridization in the same human blastomeres for preimplantation genetic diagnosis Jiaen Liu, M.D., Ph.D.,

Preimplantation genetic testing: polar bodies, blastomeres, trophectoderm cells, or blastocoelic fluid? M. Cristina Magli, M.Sc., Alessandra Pomante,

In vitro blastocyst formation of human oocytes obtained from unstimulated and stimulated cycles after vitrification at various maturational stages Hyung.

Complex chromosomal rearrangements in infertile males: complexity of rearrangement affects spermatogenesis Ji Won Kim, M.D., Eun Mi Chang, M.D., Seung-Hun.

Separation of a pronucleus by premature cytokinesis: a mechanism for immediate diploidization of tripronuclear oocytes? Bernd E. Rosenbusch, Ph.D., Michael.

Pregnancy and live birth following the transfer of vitrified–warmed blastocysts derived from zona- and corona-cell-free oocytes Yimin Shu, Wenlin Peng,

Multinucleation in normally fertilized embryos is associated with an accelerated ovulation induction response and lower implantation and pregnancy rates.

Preimplantation genetic diagnosis (PGD) for extremes—successful birth after PGD for a consanguineous couple carrying an identical balanced reciprocal.

Moving to blastocyst biopsy for preimplantation genetic diagnosis and single embryo transfer at sydney IVF Kylie A de Boer, Ph.D., James W Catt, Ph.D.,

The effect of blastomere biopsy on preimplantation mouse embryo development and global gene expression Francesca E. Duncan, Ph.D., Paula Stein, Ph.D.,

Fast multicolor primed in situ protocol for chromosome identification in isolated cells may be used for human oocytes and polar bodies Franck Pellestor,

Crypt Y chromosome fragment resulting from an X;Y translocation in a patient with premature ovarian failure De-Hua Cheng, M.Sc., Yue-Qiu Tan, Ph.D.,

Aneuploidies of chromosomes 1, 4, and 6 are not compatible with human embryos' implantation M. Cristina Magli, M.Sc., Luca Gianaroli, M.D., Andor Crippa,

Complex chromosomal rearrangements in infertile males: complexity of rearrangement affects spermatogenesis Ji Won Kim, M.D., Eun Mi Chang, M.D., Seung-Hun.

Luis A. Parada, Philip G. McQueen, Peter J. Munson, Tom Misteli

Initial IVF-ET experience with assisted hatching performed 3 days after retrieval followed by day 5 embryo transfer Margaret C. Graham, M.S., Kathleen.

P Colls, N Goodall, X Zheng, S Munné Reproductive BioMedicine Online

Application of three-dimensional fluorescence in situ hybridization to human preimplantation genetic diagnosis Li-Ying Yan, Ph.D., Jie Qiao, M.D., Yuan.

Is the resulting phenotype of an embryo with balanced X-autosome translocation, obtained by means of preimplantation genetic diagnosis, linked to the.

Single nucleotide polymorphism microarray–based concurrent screening of 24- chromosome aneuploidy and unbalanced translocations in preimplantation human.

Prediction of embryo developmental potential and pregnancy based on early stage morphological characteristics Peter Sjöblom, Ph.D., Judith Menezes, Ph.D.,

Proof of concept: preimplantation genetic screening without embryo biopsy through analysis of cell-free DNA in spent embryo culture media Mousa I. Shamonki,

Conversion and non-conversion approach to preimplantation diagnosis for chromosomal rearrangements in 475 cycles Anver Kuliev, Jeanine Cieslak Janzen,

Premature ovarian failure in a patient with a complex chromosome rearrangement involving the critical region Xq24, characterized by analysis using fluorescence.

Hydrosalpinx fluid affects murine embryonic development in a coculture system with epithelial endometrial cells Isabel Carrasco, Elisa Cebral, Ph.D.,

Normal birth after single-embryo transfer in a patient with excessive polypronuclear zygote formation following in vitro fertilization and intracytoplasmic.

Differential pH in embryo culture

Cryopreservation of human germinal vesicle stage and in vitro matured M II oocytes: influence of cryopreservation media on the survival, fertilization,

Messenger RNA expression for the follicle-stimulating hormone receptor and luteinizing hormone receptor in human oocytes and preimplantation-stage embryos

Assessing the true incidence of mosaicism in preimplantation embryos

Dichorionic triamniotic triplet pregnancy with monozygotic twins discordant for trisomy 13 after preimplantation genetic screening: case report Deborah.

The nature and origin of binucleate cells in human preimplantation embryos: relevance to placental mesenchymal dysplasia L. Xanthopoulou, J.D.A. Delhanty,

Nitric oxide metabolite production in the human preimplantation embryo and successful blastocyst formation Christopher W. Lipari, M.D., Jairo E. Garcia,

Accuracy of FISH analysis in predicting chromosomal status in patients undergoing preimplantation genetic diagnosis Catherine M. DeUgarte, M.D., Man.

Incidence of chromosomal mosaicism in morphologically normal nonhuman primate preimplantation embryos Cathérine Dupont, Ph.D., James Segars, M.D., Alan.

Hideyuki Nakano, M.D., Ph.D., Harumi Kubo, M.D., Ph.D.

Human embryo twinning with applications in reproductive medicine

William B. Schoolcraft, M. D. , Elpida Fragouli, Ph. D

Selective insulin-like growth factor-I antagonist inhibits mouse embryo development in a dose-dependent manner Jose Inzunza, Ph.D., Olle Danielsson,

Comparison of urinary and recombinant follicle-stimulating hormone in in vitro growth, maturation, and fertilization of mouse preantral follicles Giannina.

Reciprocal translocation t(7;16)(q21.2;p13.3) in an infertile man

Karyotyping of human metaphase II oocytes by multifluor fluorescence in situ hybridization Julie M Clyde, Ph.D., Jan E Hogg, M.Sc., Anthony J Rutherford,

Comprehensive chromosome screening is highly predictive of the reproductive potential of human embryos: a prospective, blinded, nonselection study Richard.

Barry Behr, Ph.D., H.C.L.D., Amin A Milki, M.D.

Stimulation of mitochondrial embryo metabolism by dichloroacetic acid in an aged mouse model improves embryo development and viability Nicole O. McPherson,

Preimplantation genetic diagnosis for a carrier of a Y;autosome translocation resulting in a healthy male offspring Caroline Mackie Ogilvie, D.Phil.,

Meiotic segregation of complex reciprocal translocations: direct analysis of the spermatozoa of a t(5;13;14) carrier Franck Pellestor, Ph.D., Jacques.

Blastocyst biopsy with comprehensive chromosome screening and fresh embryo transfer significantly increases in vitro fertilization implantation and delivery.

Occurrence and developmental consequences of vacuoles throughout preimplantation development Thomas Ebner, Ph.D., Marianne Moser, Ph.D., Michael Sommergruber,

Day 3 morphology is a poor predictor of blastocyst quality in extended culture James Graham, M.S., Taer Han, B.S., Richard Porter, Michael Levy, M.D.,

Aneuploidy screening in a coelomic sample from a missed abortion using sequential fluorescence in situ hybridization Katerina Chatzimeletiou, Ph.D.,

Is the resulting phenotype of an embryo with balanced X-autosome translocation, obtained by means of preimplantation genetic diagnosis, linked to the.

Preimplantation diagnosis for aneuploidies in patients undergoing in vitro fertilization with a poor prognosis: identification of the categories for which.

An improved fixation technique for fluorescence in situ hybridization for preimplantation genetic diagnosis Dmitri I Dozortsev, M.D., Ph.D., Kevin T.

Where does the embryo implant after embryo transfer in humans?

In vitro development and chromosome constitution of embryos derived from monopronucleated zygotes after intracytoplasmic sperm injection Sílvia Mateo,

Presentation transcript:

Effect of chromosomal translocations on the development of preimplantation human embryos in vitro S Evsikov, Ph.D., J Cieslak, Y Verlinsky, Ph.D. Fertility and Sterility Volume 74, Issue 4, Pages 672-677 (October 2000) DOI: 10.1016/S0015-0282(00)01513-2

Figure 1 Metaphase plate from tetraploid heterokaryon between human blastomere and mouse zygote. Translocation case t(1;8). (A), Phase-contrast view of a diploid human metaphase (arrow) and two haploid mouse metaphases (arrowheads) immediately after heterokaryon fixation. (B), Whole chromosome painting (WCP). Chromosome 1 painted green; chromosome 8, orange. No definite conclusion can be drawn from this metaphase as to the presence of a translocation: three out of four chromosomes involved overlap. (C), The same embryo was biopsied on day 4 of development. Phase-contrast view of the metaphase after Pepsin treatment. (D), WCP. Chromosome 1 painted green; chromosome 8, orange. Metaphase has no overlaps. The embryo was determined to be normal. Evsikov. Chromosome translocations and embryo development. Fertil Steril 2000. Fertility and Sterility 2000 74, 672-677DOI: (10.1016/S0015-0282(00)01513-2)

Figure 2 Translocation case t(12;18). Metaphase plates obtained from a single blastomere of a 10-cell embryo and the respective blastocyst fixed on day 6 of development (200+ cell stage). Whole chromosome painting. Arrows indicate der(12). (A), Blastomere nucleus conversion shows [der(12), 18, 18]: chromosome 12 painted green; chromosome 18, orange; centromeric probe for chromosome 18, aqua. (B–D) Metaphases obtained from the same embryo at the blastocyst stage show [12, der(12), 18, 18]. Chromosome 12 painted green; chromosome 18, orange. Evsikov. Chromosome translocations and embryo development. Fertil Steril 2000. Fertility and Sterility 2000 74, 672-677DOI: (10.1016/S0015-0282(00)01513-2)