IgE+ cells in the peripheral blood of atopic, nonatopic, and bee venom–hypersensitive individuals exhibit the phenotype of highly differentiated B cells

Slides:

Advertisements

Similar presentations

Superior biologic activity of the recombinant bee venom allergen hyaluronidase expressed in baculovirus-infected insect cells as compared with Escherichia.

Advertisements

Panimmunoglobulin and IgE-selective extracorporeal immunoadsorption in patients with severe atopic dermatitis Kristian Reich, MD, PhD, Julia Deinzer,

Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy Erik Wambre, PhD, Jonathan.

Daniela P. Metz, PhDa, Annette S

The loss of IgM memory B cells correlates with clinical disease in common variable immunodeficiency Rita Carsetti, MD, Maria Manuela Rosado, PhD, Simona.

Expression of FcγRIII (CD16) on human peripheral blood eosinophils increases in allergic conditions Francis Davoine, MSc, Sophie Lavigne, MSc, Jamila.

Expression of IL-9 receptor α chain on human germinal center B cells modulates IgE secretion Lama M. Fawaz, PhD, Ehssan Sharif-Askari, PhD, Oumnia Hajoui,

Establishment of human IgE system in severe combined immunodeficient mice with peripheral blood mononuclear cells from asthmatic children Bor-Luen Chiang,

Different expression of cytokine and membrane molecules by circulating lymphocytes on acute mental stress in patients with atopic dermatitis in comparison.

Tan Jinquan, MD, PhD, Henrik H. Jacobi, MD, Chen Jing, MD, Claus M

Reduced endothelial progenitor cells in extracranial arterial stenosis but not intracranial arterial stenosis Zhixin Huang, MD, PhD, Xiaohao Zhang, MD,

Wheat α-amylase inhibitor: A second route of allergic sensitization

Predominance of type 2 cytokine–producing CD4+ and CD8+ cells in patients with atopic dermatitis Masatoshi Nakazawa, DVMa, Nakako Sugi, MDb, Hiroshi.

Immunologic defects in 22q11.2 deletion syndrome

Toll-like receptor 7–induced naive human B-cell differentiation and immunoglobulin production Mark C. Glaum, MD, PhD, Shilpi Narula, MD, Decheng Song,

The inhibitory effects of topically active glucocorticoids on IL-4, IL-5, and interferon-γ production by cultured primary CD4+ T cells Shelby P. Umland,

Magdalena A. Berkowska, PhD, Jorn J

Evidence for a role for IL-5 and eotaxin in activating and recruiting eosinophils in drug- induced cutaneous eruptions Nikhil Yawalkar, MD, Maithili Shrikhande,

FOXP3+ CD4 T-cell maturity and responses to microbial stimulation alter with age and associate with early-life gut colonization Sophia Björkander, Maria.

Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis Kaori Mukai, PhD, Nicolas Gaudenzio,

Omalizumab treatment downregulates dendritic cell FcεRI expression

Allergen-specific IgE production of committed B cells from allergic patients in vitro Peter Steinberger, MSca, Barbara Bohlea, Franco di Padova, MDb,

Hydroxychloroquine improves airflow and lowers circulating IgE levels in subjects with moderate symptomatic asthma B.Lauren Charous, MD, Elkan F. Halpern,

Disialoganglioside GD3 is selectively expressed by developing and mature human mast cells Shunlin Ren, PhDa, Naotomo Kambe, MDa, Zhongmin Du, PhDa, Yongli.

Human IL-31 is induced by IL-4 and promotes TH2-driven inflammation

Kathleen R. Bartemes, BA, Gail M. Kephart, BS, Stephanie J

T-cell receptor diversity is selectively skewed in T-cell populations of patients with Wiskott-Aldrich syndrome Junfeng Wu, MD, Dawei Liu, MS, Wenwei.

Differential expression of functional chemokine receptors on human blood and lung group 2 innate lymphoid cells Cathryn A. Weston, PhD, Batika M.J. Rana,

Panimmunoglobulin and IgE-selective extracorporeal immunoadsorption in patients with severe atopic dermatitis Kristian Reich, MD, PhD, Julia Deinzer,

Human mast cells are major IL-22 producers in patients with psoriasis and atopic dermatitis Shunya Mashiko, BPharm, Salim Bouguermouh, MD, PhD, Manuel.

Differential immune responses to acute lower respiratory illness in early life and subsequent development of persistent wheezing and asthma Fernando.

Richard M. Siegel, MD, PhD, Thomas A. Fleisher, MD

Brent E. Palmer, PhD, Douglas G. Mack, PhD, Allison K

Impact of immunotherapy on blood dendritic cells in patients with Hymenoptera venom allergy Katharina Dreschler, Kai Bratke, PhD, Sebastian Petermann,

IgE autoantibodies monitored in a patient with atopic dermatitis under cyclosporin A treatment reflect tissue damage Tamar Kinaciyan, MD a*, Susanne.

Direct exposure of carpets to sunlight can kill all mites

Inflammatory cells, cytokine and chemokine expression in asthma immunocytochemistry and in situ hybridization Qutayba Hamid, MD, PhD, Editor Journal.

Expression of IL-9 receptor α chain on human germinal center B cells modulates IgE secretion Lama M. Fawaz, PhD, Ehssan Sharif-Askari, PhD, Oumnia Hajoui,

Daniela P. Metz, PhDa, Annette S

Cell surface characterization of T lymphocytes and allergen-specific T cell clones: Correlation of CD26 expression with T H1 subsets Martin Willheim,

Heterogeneity of mast cells and T cells in the nasal mucosa

Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy Erik Wambre, PhD, Jonathan.

Barbara Foster, MSa, Lawrence B

Molecular virology and immunology of HIV infection

Ganglioside GQ1b enhances Ig production by human PBMCs

Lisa D. Vailes, MSa, Michael T. Kinter, PhDb, L

Classification of anti-FcϵRI and anti-IgE autoantibodies in chronic idiopathic urticaria and correlation with disease severity Ruth A. Sabroe, MDa, Edda.

Evaluation of devices for skin prick testing

IL-9 expression by human eosinophils: Regulation by IL-1β and TNF-α

Healthy subjects express differences in clinical responses to inhaled lipopolysaccharide that are related with inflammation and with atopy Olivier Michel,

The discovery of IgE Journal of Allergy and Clinical Immunology

Sara Paveglio, PhD, MS, Erin Bennett, MS, Kelly L. Hawley, PhD, Adam P

Fractional analysis of sequential induced sputum samples during sputum induction: Evidence that different lung compartments are sampled at different time.

Intracellular IL-5 and T-lymphocyte subsets in atopic and nonatopic bronchial asthma Yutaro Shiota, MD, PhDa, Hitomi Arikita, MTa, Naokatsu Horita, MDb,

Harald Renz, MD, Chaya Brodie, PhD, Katherine Bradley, BS, Donald Y. M

Defining antigen-specific responses with human MHC class II tetramers

Inhibition of human allergic T-cell responses by IL-10–treated dendritic cells: Differences from hydrocortisone-treated dendritic cells Iris Bellinghausen,

Acquisition and alteration of adhesion molecules during cultured human mast cell differentiation Hiroshi Tachimoto, MD, PhD, Sherry A. Hudson, MSB, Bruce.

Highly increased levels of IgE antibodies to vaccine components in children with influenza vaccine–associated anaphylaxis Mizuho Nagao, MD, PhD, Takao.

Perforin hyperreleasability and depletion in cytotoxic T cells from patients with exacerbated atopic dermatitis and asymptomatic rhinoconjunctivitis allergica

Joanna J. Lukawska, MD, Lefteris Livieratos, PhD, Barbara M

Gp120- and TNF-α–induced modulation of human B cell function: Proliferation, cyclic AMP generation, Ig production, and B-cell receptor expression Christina.

Retinoic acid modulates IL-5 receptor expression and selectively inhibits eosinophil- basophil differentiation of hemopoietic progenitor cells John W.

Human BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy Rodrigo Jiménez-Saiz, PhD, Yosef Ellenbogen, BHSc, Kelly Bruton, BSc,

Local expression of ϵ germline gene transcripts and RNA for the ϵ heavy chain of IgE in the bronchial mucosa in atopic and nonatopic asthma Sun Ying,

Reply Journal of Allergy and Clinical Immunology

IL-4– and IL-5–positive T lymphocytes, eosinophils, and mast cells in allergen-induced late-phase cutaneous reactions in atopic subjects Luis T. Barata,

Comparison of ciliary activity and inflammatory mediator release from bronchial epithelial cells of nonatopic nonasthmatic subjects and atopic asthmatic.

IgG4 production is confined to human IL-10–producing regulatory B cells that suppress antigen-specific immune responses Willem van de Veen, MSc, Barbara.

Characterization of dendritic cells from human oral mucosa: A new Langerhans' cell type with high constitutive FcϵRI expression Jean-Pierre Allam, MDa,

Presentation transcript:

IgE+ cells in the peripheral blood of atopic, nonatopic, and bee venom–hypersensitive individuals exhibit the phenotype of highly differentiated B cells Peter J. Donohoe, PhDa, Robert J. Heddle, MD, PhD a, Pamela J. Sykes, PhDb, Michael Fusco, BSca, Loretta R. Flego, BSca, Heddy Zola, PhDa Journal of Allergy and Clinical Immunology Volume 95, Issue 2, Pages 587-596 (February 1995) DOI: 10.1016/S0091-6749(95)70321-7 Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 1 Reduction of adsorbtion of immunoglobulin to lymphocytes. Cells were incubated at room temperature for 30 minutes with neat normal sheep serum. Data are presented on PBLs from an atopic donor (A) without and (B) with preincubation. Journal of Allergy and Clinical Immunology 1995 95, 587-596DOI: (10.1016/S0091-6749(95)70321-7) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 1 Reduction of adsorbtion of immunoglobulin to lymphocytes. Cells were incubated at room temperature for 30 minutes with neat normal sheep serum. Data are presented on PBLs from an atopic donor (A) without and (B) with preincubation. Journal of Allergy and Clinical Immunology 1995 95, 587-596DOI: (10.1016/S0091-6749(95)70321-7) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 2 Gating parameters used to analyze lymphocytes (R1) from 10,000 PBLs (upper left). IgE fluorescence distribution in the lymphocyte population (R1) (upper right). Real-time gating histogram showing the range for data collection (R2) and the gating (R3) used for data analysis (center left). Dot plot of expression of kappa and lambda light chains on IgE real-time–gated cells with gate R1 and the brightly fluorescent population R3 (center right). At lower left and right are dot plots showing kappa and lambda expression on the less brightly stained populations in R4 and R5. Journal of Allergy and Clinical Immunology 1995 95, 587-596DOI: (10.1016/S0091-6749(95)70321-7) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 2 Gating parameters used to analyze lymphocytes (R1) from 10,000 PBLs (upper left). IgE fluorescence distribution in the lymphocyte population (R1) (upper right). Real-time gating histogram showing the range for data collection (R2) and the gating (R3) used for data analysis (center left). Dot plot of expression of kappa and lambda light chains on IgE real-time–gated cells with gate R1 and the brightly fluorescent population R3 (center right). At lower left and right are dot plots showing kappa and lambda expression on the less brightly stained populations in R4 and R5. Journal of Allergy and Clinical Immunology 1995 95, 587-596DOI: (10.1016/S0091-6749(95)70321-7) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 2 Gating parameters used to analyze lymphocytes (R1) from 10,000 PBLs (upper left). IgE fluorescence distribution in the lymphocyte population (R1) (upper right). Real-time gating histogram showing the range for data collection (R2) and the gating (R3) used for data analysis (center left). Dot plot of expression of kappa and lambda light chains on IgE real-time–gated cells with gate R1 and the brightly fluorescent population R3 (center right). At lower left and right are dot plots showing kappa and lambda expression on the less brightly stained populations in R4 and R5. Journal of Allergy and Clinical Immunology 1995 95, 587-596DOI: (10.1016/S0091-6749(95)70321-7) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 2 Gating parameters used to analyze lymphocytes (R1) from 10,000 PBLs (upper left). IgE fluorescence distribution in the lymphocyte population (R1) (upper right). Real-time gating histogram showing the range for data collection (R2) and the gating (R3) used for data analysis (center left). Dot plot of expression of kappa and lambda light chains on IgE real-time–gated cells with gate R1 and the brightly fluorescent population R3 (center right). At lower left and right are dot plots showing kappa and lambda expression on the less brightly stained populations in R4 and R5. Journal of Allergy and Clinical Immunology 1995 95, 587-596DOI: (10.1016/S0091-6749(95)70321-7) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 2 Gating parameters used to analyze lymphocytes (R1) from 10,000 PBLs (upper left). IgE fluorescence distribution in the lymphocyte population (R1) (upper right). Real-time gating histogram showing the range for data collection (R2) and the gating (R3) used for data analysis (center left). Dot plot of expression of kappa and lambda light chains on IgE real-time–gated cells with gate R1 and the brightly fluorescent population R3 (center right). At lower left and right are dot plots showing kappa and lambda expression on the less brightly stained populations in R4 and R5. Journal of Allergy and Clinical Immunology 1995 95, 587-596DOI: (10.1016/S0091-6749(95)70321-7) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 2 Gating parameters used to analyze lymphocytes (R1) from 10,000 PBLs (upper left). IgE fluorescence distribution in the lymphocyte population (R1) (upper right). Real-time gating histogram showing the range for data collection (R2) and the gating (R3) used for data analysis (center left). Dot plot of expression of kappa and lambda light chains on IgE real-time–gated cells with gate R1 and the brightly fluorescent population R3 (center right). At lower left and right are dot plots showing kappa and lambda expression on the less brightly stained populations in R4 and R5. Journal of Allergy and Clinical Immunology 1995 95, 587-596DOI: (10.1016/S0091-6749(95)70321-7) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 3 Histograms of IgE+ cells after gating on the lymphocyte population. Examples from each of the donor groups are shown with data from 100,000 cells. IgE population was selected with the criteria outlined in the legend to Fig. 2. Journal of Allergy and Clinical Immunology 1995 95, 587-596DOI: (10.1016/S0091-6749(95)70321-7) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 3 Histograms of IgE+ cells after gating on the lymphocyte population. Examples from each of the donor groups are shown with data from 100,000 cells. IgE population was selected with the criteria outlined in the legend to Fig. 2. Journal of Allergy and Clinical Immunology 1995 95, 587-596DOI: (10.1016/S0091-6749(95)70321-7) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 3 Histograms of IgE+ cells after gating on the lymphocyte population. Examples from each of the donor groups are shown with data from 100,000 cells. IgE population was selected with the criteria outlined in the legend to Fig. 2. Journal of Allergy and Clinical Immunology 1995 95, 587-596DOI: (10.1016/S0091-6749(95)70321-7) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 4 Direct enumeration of IgE+ cells in PBLs from each of the three donor groups. Filled circles, Cell count carried out on 100,000 cells, n = 3; open circles, cell count carried out on 10,000 cells, n = 20 (see text). Journal of Allergy and Clinical Immunology 1995 95, 587-596DOI: (10.1016/S0091-6749(95)70321-7) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 5 Histograms showing the percent expression of CD19, CD38, CD32, CD45RO, and CD45RA on cells collected by real-time gating on the IgE+ lymphocyte population. The lower right histogram shows expression of IgE on PLA-2+ population. The negative control (×63) is superimposed on each graph (unshaded area). Journal of Allergy and Clinical Immunology 1995 95, 587-596DOI: (10.1016/S0091-6749(95)70321-7) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 5 Histograms showing the percent expression of CD19, CD38, CD32, CD45RO, and CD45RA on cells collected by real-time gating on the IgE+ lymphocyte population. The lower right histogram shows expression of IgE on PLA-2+ population. The negative control (×63) is superimposed on each graph (unshaded area). Journal of Allergy and Clinical Immunology 1995 95, 587-596DOI: (10.1016/S0091-6749(95)70321-7) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 5 Histograms showing the percent expression of CD19, CD38, CD32, CD45RO, and CD45RA on cells collected by real-time gating on the IgE+ lymphocyte population. The lower right histogram shows expression of IgE on PLA-2+ population. The negative control (×63) is superimposed on each graph (unshaded area). Journal of Allergy and Clinical Immunology 1995 95, 587-596DOI: (10.1016/S0091-6749(95)70321-7) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 6 Expression of CD38 on IgE + cells with three-color staining. Top, Histogram of CD3 expression on lymphocytes triple-stained with anti-IgE, CD38, and CD3. Region R2 shows the gating for collection of cells, and R3 shows the gating used to generate the dot plot in the bottom panel. Bottom, Dot plot of cells stained with OKT10 and IgE after gating out the CD3-negative population. Quadrants set for IgE+ parameters were selected by the generation of a histogram as described in the legend to Fig. 2. Journal of Allergy and Clinical Immunology 1995 95, 587-596DOI: (10.1016/S0091-6749(95)70321-7) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 6 Expression of CD38 on IgE + cells with three-color staining. Top, Histogram of CD3 expression on lymphocytes triple-stained with anti-IgE, CD38, and CD3. Region R2 shows the gating for collection of cells, and R3 shows the gating used to generate the dot plot in the bottom panel. Bottom, Dot plot of cells stained with OKT10 and IgE after gating out the CD3-negative population. Quadrants set for IgE+ parameters were selected by the generation of a histogram as described in the legend to Fig. 2. Journal of Allergy and Clinical Immunology 1995 95, 587-596DOI: (10.1016/S0091-6749(95)70321-7) Copyright © 1995 Mosby, Inc. Terms and Conditions