A–E: Adiponectin secretion is stimulated via adrenergic pathways in a Ca2+-independent manner. A–E: Adiponectin secretion is stimulated via adrenergic.

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A–E: Adiponectin secretion is stimulated via adrenergic pathways in a Ca2+-independent manner. A–E: Adiponectin secretion is stimulated via adrenergic pathways in a Ca2+-independent manner. A: Adiponectin secretion in 3T3-L1 adipocytes expressed as a fold increase compared with the basal level (5 mmol/L glucose) during 30-min incubations with epinephrine (EPI; 5 μmol/L) or CL (1 μmol/L) with or without BAPTA pretreatment (50 μmol/L for 30 min). B–D: mRNA expression in undifferentiated (day 0; U) and differentiated (day 8; D) 3T3-L1 adipocytes. The PCR efficiency (log4 dilutions; starting from 5 ng RNA/reaction) was as follows: Actb 97.6%, Adra1d 97.8%, Adrb1 95.6%, Adrb2 103.6%, and Adrb3 102.1%. E: CL-stimulated adiponectin secretion (30-min incubations) in 3T3-L1 adipocytes preincubated in the absence or presence of cycloheximide (CHX; 10 µg/mL for 30 min). Data represent 8–13 experiments in A and E and 6 experiments (3 undifferentiated and 3 differentiated) in B–D. *P < 0.05; **P < 0.01; ***P < 0.001 vs. control. Ali M. Komai et al. Diabetes 2016;65:3301-3313 ©2016 by American Diabetes Association