Effects of Chd1-knockdown on global gene expression.

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Effects of Chd1-knockdown on global gene expression. Effects of Chd1-knockdown on global gene expression. (A) Immunofluorescence detection of BrUTP incorporation as a measure of genome-wide transcription in control and Chd1-knockdown embryos (red, BrUTP; blue, chromatin). Twenty embryos were used in each experiment (n=3): in total, 60 embryos each for siControl and siChd1. (B) Quantitation of fluorescence signal intensity for BrUTP incorporation assay. The signal intensity of BrUTP in the nuclei was individually quantitated. The signal intensity of the nuclei in control embryos was defined as 1. Data are expressed as means±s.e.m. (n=15). Shinnosuke Suzuki et al. Development 2015;142:2375-2384 © 2015. Published by The Company of Biologists Ltd