hiPSC-CMs exhibit sarcomeric proteins and express human RTK families

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Fig. 1. hiPSC-CMs exhibit sarcomeric proteins and express human RTK families. hiPSC-CMs exhibit sarcomeric proteins and express human RTK families. (A) Diagram of study workflow. Somatic tissue samples were obtained from 13 individuals and reprogrammed into hiPSC colonies with either Sendai virus or lentivirus vectors expressing the transcription factors OCT4, SOX2, KLF4, and MYC (OKSM). hiPSCs were differentiated into hiPSC-CMs, hiPSC-ECs, and hiPSC-CFs. Purified cardiomyocytes were treated with TKIs and examined for alterations in cell viability, contractility, cellular signaling, and gene expression. (B) Confocal microscopic immunofluorescence images of differentiated hiPSC-CMs expressing the sarcomeric markers cardiac troponin T (TNNT2) and α-actinin (ACTN2). DAPI, 4′,6-diamidino-2-phenylindole. (C) Day 30 hiPSC-CMs from five healthy control lines expressing major RTKs including INSR, IGF1R, PDGFRA, and KDR. High TNNT2 and low PECAM1 expression indicates a pure hiPSC-CM population devoid of ECs. n = 3 biological replicates conducted for gene expression analysis in each hiPSC-CM line. Data are means ± SEM. RPKM, reads per kilobase per million. Arun Sharma et al., Sci Transl Med 2017;9:eaaf2584 Copyright © 2017, American Association for the Advancement of Science