Loss of Graf results in plasmatocyte overproliferation.

Slides:

Advertisements

Similar presentations

Héctor Herranz, Ruifen Weng, Stephen M. Cohen Current Biology

Advertisements

Phenotypic analysis of spo73∆ mutant.

Volume 16, Issue 5, Pages (May 2009)

Volume 6, Issue 3, Pages (September 2009)

Yorkie and Scalloped Signaling Regulates Notch-Dependent Lineage Specification during Drosophila Hematopoiesis Gabriel B. Ferguson, Julian A. Martinez-Agosto

Mutual Repression by Bantam miRNA and Capicua Links the EGFR/MAPK and Hippo Pathways in Growth Control Héctor Herranz, Xin Hong, Stephen M. Cohen Current.

Bernard Duvic, Jules A Hoffmann, Marie Meister, Julien Royet

Volume 22, Issue 10, Pages (March 2018)

BTB/POZ-Zinc Finger Protein Abrupt Suppresses Dendritic Branching in a Neuronal Subtype-Specific and Dosage-Dependent Manner Wenjun Li, Fay Wang, Laurent.

The Snail Family Member Worniu Is Continuously Required in Neuroblasts to Prevent Elav-Induced Premature Differentiation Sen-Lin Lai, Michael R. Miller,

Gain and loss of Cdk5α induces overt neurodegeneration and degenerative phenotypes. Gain and loss of Cdk5α induces overt neurodegeneration and degenerative.

Volume 6, Issue 3, Pages (September 2009)

Percentage fractions of (β-Gal+) PCs in 32-week-old heterozygous females differ between cerebellar lobules affected and spared from degeneration of Purkinje.

Héctor Herranz, Ruifen Weng, Stephen M. Cohen Current Biology

ILK knockdown decreases mTOR signaling in PKD kidneys.

Tapetal mitochondria in wild-type (WT), drp3b, and elm1 mutants.

Inducible DM1 model displays increased autophagy.

Recruitment of PH-Akt-GFP to the leading edge of the cell during chemotaxis. Recruitment of PH-Akt-GFP to the leading edge of the cell during chemotaxis.

Fig. 1. Overview of the nervous system of the adult S. roscoffensis.

VacA does not induce phosphorylation of Src in RPTPα constitutive-knockdown AZ-521 cells. VacA does not induce phosphorylation of Src in RPTPα constitutive-knockdown.

A highly conserved six-amino-acid region in the C-terminal CT domain of MARCH8 is responsible for its ability to downregulate TfR. A highly conserved six-amino-acid.

Dany is required for normal spermatogenesis to proceed beyond the spermatocyte stage. dany is required for normal spermatogenesis to proceed beyond the.

Smo deficiency in the renal stroma is dependent on GLI3R.

FGF/ERK inhibition accelerates neural fate conversion of EpiSCs.

Fig. 2. Salivary glands from RasV12-expressing larvae produce MMP1, release tissue fragments into the hemolymph and express apoptotic markers.Salivary.

DHR3 suppresses Phm protein levels.

The initial targeting of Sec61b mRNA to the ER is partially dependent on ribosomes and translation. The initial targeting of Sec61b mRNA to the ER is partially.

Fcp1 CTD phosphatase affects a broad range of transcripts in the +N and -N media. Fcp1 CTD phosphatase affects a broad range of transcripts in the +N and.

Hipk induces hemocyte-derived melanotic tumors.

Fitm RNAi-1 knockdown in Drosophila interferes with morphology of nociceptive multi-dendritic sensory neurons. Fitm RNAi-1 knockdown in Drosophila interferes.

Fig. 6. Comparison of Plk4 with Sas-6 localization

Fig. 6. Effect of SAHA and ML on histone acetylation, BAX, and p21CDKN1A expression.PANC-1 and BxPC-3 cells were incubated for 48 hours with 5 µM.

Delayed formation of sensory hair cells in dlx3b/4b-deficient embryos

Overexpression of individual signaling pathway components does not phenocopy the cell-spreading phenotype induced by elevated Hipk. Overexpression of individual.

Fig. 8. The morphology of the ventral nerve cord in Ror-Myc overexpressing embryos is normal. The morphology of the ventral nerve cord in Ror-Myc overexpressing.

Loss of LAR phosphatase activity is associated with decreased adhesion complex formation. Loss of LAR phosphatase activity is associated with decreased.

TWEAK increases histone crotonylation in kidney tubular cells.

Rgs16::GFP expression in pancreatic neoplasia.

ArfGAP1 expression alters GBF1 recruitment to Golgi membranes.

Transbilayer distribution of cholesterol in the plasma membrane is defective in staurosporine-treated cells. Transbilayer distribution of cholesterol in.

EpiDEG efficiently degrades GFP-tagged proteins that localize to different subcellular localizations. epiDEG efficiently degrades GFP-tagged proteins that.

The SIM domain of RAD51 is required for HR

Fgfr3;4 mutant lungs display an increase in Mfap5, Igf1 and Fbn2 expression. Fgfr3;4 mutant lungs display an increase in Mfap5,Igf1and Fbn2 expression.

Ibm1 and edm2 mutants generate more stomatal divisions in the leaf epidermis. ibm1 and edm2 mutants generate more stomatal divisions in the leaf epidermis.

Prophase nuclear movements in wild-type, rec8 and rec7 mutant cells.

Expression of active Rho partially rescues Erk1/2 activation and peripheral FA phenotype in RIAM-knockdown cells. Expression of active Rho partially rescues.

Lysine residues in the cytoplasmic region of TfR are involved in the MARCH8-induced downregulation of TfR. Lysine residues in the cytoplasmic region of.

Graf-dependent GEEC endocytosis is required for EGFR internalization and degradation at high Spi. Graf-dependent GEEC endocytosis is required for EGFR.

Global cell proliferation replenishes epidermal cells.

The zebrafish swdp75fm and swdp82mf mutations cause exocrine pancreatic hypoplasia and reduced skin pigmentation. The zebrafish swdp75fm and swdp82mf mutations.

A high-sugar diet induces insulin resistance.

Fig. 2. Acetylation stiffens primary cilia.

Fig. 1. Cell adhesion molecule expression and the aggregation of wildtype and mutant ES cells.(A) Wildtype (WT), E-cadherin null (9J), and N-cadherin null.

Fig. 5. Testis defects in STK35 KO mice.

The BRCA1 aggregates exclude large nuclear structures.

Integrin-mediated signaling is required for PFC maturation.

WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates TfR. WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates.

Failure of chromosome disassembly in mip120 null ovarian nurse cells

Histone crotonylation in kidney tubular cells.

Fig. 7. Analysis of dFMRP kinetics in dFMRP granules by FRAP

The absence of host Fbln5 results in increased apoptosis and decreased proliferation in Pan02 tumors. The absence of host Fbln5 results in increased apoptosis.

Lack of Ctip2 is associated with impaired proliferation and delayed onset of differentiation during early stages of epidermal development. Lack of Ctip2.

Maytansinol disrupts the mitotic spindle and prevents mitotic exit in Drosophila. Maytansinol disrupts the mitotic spindle and prevents mitotic exit inDrosophila.

Subnuclear localization of HA-tagged EBNA1 protein.

Diaphanous is required presynaptically for normal synaptic growth.

SlMYB21 Encodes an Active Transcription Factor That Interacts With SlJAZ9 and Complements the Arabidopsis Mutant myb21-5. SlMYB21 Encodes an Active Transcription.

Rab3 Dynamically Controls Protein Composition at Active Zones

Subperineurial glia are required for normal GluRIIA distribution.

GATOR1 is required to maintain baseline levels of TORC1 activity in Drosophila. GATOR1 is required to maintain baseline levels of TORC1 activity in Drosophila.

Cellular localization of Panx1 and altered membrane morphology in stable Panx1-transfected C6 glioma cells. Cellular localization of Panx1 and altered.

Presentation transcript:

Loss of Graf results in plasmatocyte overproliferation. Loss of Graf results in plasmatocyte overproliferation. (A) Representative confocal images of hemolymph smears from wild-type (w1118) and Graf1/Df third instar larvae. Each hemolymph sample was mixed with an equal volume of DAPI solution to visualize hemocyte nuclei. (B) Concentration of circulating hemocytes in third instar larvae of the following genotypes: wild type, Graf1/Df, crq-GAL4/+ and Graf1/Df; crq- GAL4/+; +/UAS-Graf-HA (Graf rescue). (C) Live mount images of wild-type, Graf1/Df and Graf1,srp-GAL4/Df; +/UAS-Graf-HA (Graf rescue) third instar larvae expressing HmlΔdsRed. (D) Concentration of circulating GFP-positive hemocytes in UAS-mCD8-GFP/+; crq-GAL4/+ (Crq-GFP+ WT), Graf1,+/Df,UAS-mCD8-GFP; crq-GAL4/+ (Crq-GFP+Graf1/Df), lz-GAL4/UAS-mCD8-GFP (Lz-GFP+ WT) and Graf1,lz-GAL4/Df,UAS-mCD8-GFP (Lz-GFP+Graf1/Df) third instar larvae. (E,F) Graf mutants show an excess of pH3-positive cells. (E) Confocal images of total (circulating and sessile) hemocytes from wild-type and Graf1/Df second instar larvae [68-72 h after egg laying (AEL)], stained with DAPI and an anti-phospho-histone H3 (pH3) antibody (arrowheads). (F) Percentage of pH3-positive hemocytes among total hemocytes is shown for the second (68-72 h AEL) and third (92-96 h AEL) instar larvae. Data are mean±s.e.m. The total number of hemolymph samples (B,D; five independent experiments) or hemocytes (F; three independent experiments) analyzed is indicated within each bar. Each hemolymph sample involves five larvae. Comparisons are made against wild type (*P<0.001). Scale bars: 50 µm in A; 200 µm in C; 10 µm in E. Sungdae Kim et al. Development 2017;144:4159-4172 © 2017. Published by The Company of Biologists Ltd