RECQL4 depletion or malfunction increases inter-kinetochore distance.

Slides:



Advertisements
Similar presentations
Smita Srivastava, Patricia S. Grace, Joel D. Ernst  Cell Host & Microbe 
Advertisements

Andreas Brown, Johannes Pospiech, Karina Eiwen, Darren J
Concordant asymmetry of regulatory markers in sibling thymocytes.
Volume 22, Issue 5, Pages (May 2012)
Volume 24, Issue 6, Pages (March 2014)
Cdk1 inhibition does not affect kinetochore capturing.
Volume 45, Issue 5, Pages (March 2012)
Poly-GR and poly-PR co-aggregate with ribosomal proteins in C9orf72 patients. Poly-GR and poly-PR co-aggregate with ribosomal proteins in C9orf72 patients.
Clathrin-Mediated Endocytosis Persists during Unperturbed Mitosis
Mechanism of Ska Recruitment by Ndc80 Complexes to Kinetochores
Volume 43, Issue 1, Pages e6 (October 2017)
Activating and Silencing the Mitotic Checkpoint through CENP-E-Dependent Activation/Inactivation of BubR1  Yinghui Mao, Ariane Abrieu, Don W. Cleveland 
Cytoplasmic poly-GR/PR inclusions resemble stress granules in vitro.
Direct fusion lines allow fluorescent visualization of plasmatocyte nuclei, the cytoplasm, or the cytoskeleton in embryos from stage (St) 8 onwards. Direct.
Verena N. Lorenz, Michael P. Schön, Cornelia S. Seitz 
Anaphase chromosome separation in extracts takes place in the absence of intact spindle poles. Anaphase chromosome separation in extracts takes place in.
Beclin‐1 depletion reduces targeting of several outer kinetochore proteins. Beclin‐1 depletion reduces targeting of several outer kinetochore proteins.
Rapid vimentin reorganisation upon BCR stimulation.
Maki Murata-Hori, Yu-li Wang  Current Biology 
Volume 9, Issue 5, Pages (November 2017)
Disrupted dynamics of early stages of differentiation perturbs neural cell fate specification. Disrupted dynamics of early stages of differentiation perturbs.
Volume 28, Issue 17, Pages e4 (September 2018)
Heidi Hehnly, Stephen Doxsey  Developmental Cell 
by Silvia Mele, Stephen Devereux, Andrea G
Volume 28, Issue 1, Pages e3 (January 2018)
Nucleolar poly-GR/PR alter nucleolar organization and inhibit translation. Nucleolar poly-GR/PR alter nucleolar organization and inhibit translation. GFP,
Smita Srivastava, Patricia S. Grace, Joel D. Ernst  Cell Host & Microbe 
Plk1 negatively regulates NuMA cortical localization by phosphorylating at its C-terminus. Plk1 negatively regulates NuMA cortical localization by phosphorylating.
Volume 17, Issue 6, Pages (March 2007)
Plk1 inhibition or depletion causes robust cortical NuMA localization in mitosis. Plk1 inhibition or depletion causes robust cortical NuMA localization.
Volume 24, Issue 6, Pages (March 2014)
AURORA-A amplification overrides the mitotic spindle assembly checkpoint, inducing resistance to Taxol  Shubha Anand, Sue Penrhyn-Lowe, Ashok R Venkitaraman 
Kinetochore Attachments Require an Interaction between Unstructured Tails on Microtubules and Ndc80Hec1  Stephanie A. Miller, Michael L. Johnson, P. Todd.
Self-Organization of MTOCs Replaces Centrosome Function during Acentrosomal Spindle Assembly in Live Mouse Oocytes  Melina Schuh, Jan Ellenberg  Cell 
HURP is an obstacle for KIF18A on K-fibers in metaphase cells.
Distinct Roles of the Chromosomal Passenger Complex in the Detection of and Response to Errors in Kinetochore-Microtubule Attachment  Julian Haase, Mary.
Volume 20, Issue 5, Pages (March 2010)
CENP-E as an Essential Component of the Mitotic Checkpoint In Vitro
Kinetochore Dynein Is Required for Chromosome Motion and Congression Independent of the Spindle Checkpoint  Zhenye Yang, U. Serdar Tulu, Patricia Wadsworth,
Volume 106, Issue 1, Pages (July 2001)
Volume 29, Issue 6, Pages (March 2008)
KIN-3 depletion leads to increased levels of centrosomal ZYG-1
Normal RB1+/− cells have elevated γH2AX foci, mitotic errors, and aneuploidy. Normal RB1+/− cells have elevated γH2AX foci, mitotic errors, and aneuploidy.
Vimentin reorganisation requires actin and microtubules.
Nuclear DNA signal is altered in Chd5-deficient NSCs.
Mitotic chromosome misalignment is independent of DNA replication.
Inhibition of ROCK activity rescues formation of polarized cysts in the absence of DDR1 signaling. Inhibition of ROCK activity rescues formation of polarized.
Timing and Checkpoints in the Regulation of Mitotic Progression
Plk1 inhibition affects the NuMA turnover at the spindle pole.
Endogenous SMN1 is not recruited to stress granules in HeLa cells after diverse stresses. Endogenous SMN1 is not recruited to stress granules in HeLa cells.
Numerical analysis of cell morphology of tapetal cells in wild type.
Fig. 6. Comparison of Plk4 with Sas-6 localization
RECQL4 is a RanGTP dependent MAP
RECQL4 is a MAP with a spindle function.
Spindle assembly checkpoint signaling.
Inhibition of DNA replication and induction of DNA damage does not cause chromosome misalignment. Inhibition of DNA replication and induction of DNA damage.
Fibroblasts from Rothmund–Thomson syndrome patients have abnormal spindle axis and more micronuclei. Fibroblasts from Rothmund–Thomson syndrome patients.
Single MTs can be resolved in subsections of SBF-SEM image stacks.
RECQL4 is required for microtubule stability.
The microtubule-binding region of RECQL4 is required for chromosome alignment. The microtubule-binding region of RECQL4 is required for chromosome alignment.
dcn1-deletion results in attenuated cohesin cleavage at anaphase
Dual Detection of Chromosomes and Microtubules by the Chromosomal Passenger Complex Drives Spindle Assembly  Boo Shan Tseng, Lei Tan, Tarun M. Kapoor,
LC8 and its binding partners display broad cellular localization.
Chd5-deficient NSCs generate excessive astrocytes at the expense of neurons. Chd5-deficient NSCs generate excessive astrocytes at the expense of neurons.
Association of NM-HA and NM-GFP with SGs is transient.
Tension develops across sister kinetochores (red) upon their bipolar attachment by spindle microtubules (black line). Tension develops across sister kinetochores.
Analysis of centromere and kinetochore function after depletion of DmSMC4. Analysis of centromere and kinetochore function after depletion of DmSMC4. (A)
GSDMD localizes to the plasma membrane and is processed during NET formation. GSDMD localizes to the plasma membrane and is processed during NET formation.
PC loss in Nhe6-null mouse cerebellum, female and male.
Heidi Hehnly, Stephen Doxsey  Developmental Cell 
Presentation transcript:

RECQL4 depletion or malfunction increases inter-kinetochore distance. RECQL4 depletion or malfunction increases inter-kinetochore distance. (A) Cycled spindles are assembled as in Fig 3C and D and incubated for an additional 10 min with or without 6 μg/ml nocodazole. Samples were fixed, spun down on coverslips, stained for a kinetochore marker Ndc80 and DAPI, and analyzed by confocal microscopy. Maximum intensity projections are shown in the upper row. Single confocal slices (lower row) were used to detect kinetochore pairs (arrow heads) for further analysis. Quantitation shows the inter-kinetochore distance (right) and the relative angles of sister kinetochore pairs (left), measured with respect to the spindle pole to pole axis. n > 30 kinetochore pairs from > 6 structures. Note that after RECQL4 depletion, sister kinetochore pairs do not align to the pole to pole axis. Scale bar, 20 μm. ****P < 0.0001; **P < 0.01; NS (not significant) P > 0.05 (t test, two-tailed). (B) Immunofluorescence staining of control (GM00323, GM01864) and Rothmund–Thomson syndrome patient (AG05013, AG18371) fibroblasts with the kinetochore marker CREST and checkpoint marker BubR1. Scale bar, 5 μm. (C) Inter-kinetochore distance was measured in metaphase cells of control (GM00323, GM01864) and Rothmund–Thomson syndrome patient (AG05013, AG18371) fibroblasts based on CREST signals for the kinetochore pairs attached to microtubules (identified by the absence of the BubR1 signal) after 3D reconstruction. (n) indicates the number of kinetochore pairs measured per fibroblast line. P < 0.001 (t test, two-tailed). Hideki Yokoyama et al. LSA 2019;2:e201800120 © 2019 Yokoyama et al.