Megakaryocyte large protrusions can be observed by intravital correlative EM. Megakaryocyte large protrusions can be observed by intravital correlative.

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Megakaryocyte large protrusions can be observed by intravital correlative EM. Megakaryocyte large protrusions can be observed by intravital correlative EM. (A) Selected images from time-lapse intravital microscopy showing three megakaryocytes expressing vWF-tdTomato (red) (M1–M3) adjacent to a sinusoid and two proplatelet-like structures (yellow and blue arrowheads) positive for glycoprotein 1b (green) within the lumen. (B) 3D model generated from a stack of TEM images of the area observed in (A). The three megakaryocytes (green with black nuclei) adjacent to the sinusoid (pink) are all extending protrusions into the sinusoid lumen (SL). (C) Single TEM image and 3D model of M3. (D) Single tomographic slices showing the IMS, MTs (red), and a centriole (yellow). (E) 3D model of the nucleus (black), MTs, and centriole. (F) Single TEM image of M1 showing the detached large protrusion. (G) Single tomographic slice from boxed region in (F) of the interface between M1 and the detached large protrusion. Bone marrow from intravital CLEM of calvarium. Scale bars represent 25 μm in (A, B); 5 μm in (C, F); and 2 μm in (D, E, G). Images representative of a total of six megakaryocytes from three independent experiments. Source data are available for this figure. Edward Brown et al. LSA 2018;1:e201800061 © 2018 Brown et al.