The Spectrum of Mitochondrial DNA Deletions is a Ubiquitous Marker of Ultraviolet Radiation Exposure in Human Skin Amanda J. Ray, Richard Turner, Osamu.

Slides:

Advertisements

Similar presentations

Quantitative Detection and Differentiation of Human Herpesvirus 6 Subtypes in Bone Marrow Transplant Patients by Using a Single Real-Time Polymerase Chain.

Advertisements

Amy M. Dworkin, Stephanie Y. Tseng, Dawn C. Allain, O

Ultraviolet B-Induced Skin Angiogenesis Is Associated with a Switch in the Balance of Vascular Endothelial Growth Factor and Thrombospondin-1 Expression

Ultraviolet Radiation Increases Tropoelastin mRNA Expression in the Epidermis of Human Skin In Vivo Jin Young Seo, Seong Hun Lee, Choon Shik Youn, Hai.

Photobiologic and Photoimmunologic Characteristics of XPA Gene-Deficient Mice Takeshi Horio, Hiroko Miyauchi-Hashimoto, Kazue Kuwamoto, Satoshi Horiki,

Inactivation of the Vitamin D Receptor Enhances Susceptibility of Murine Skin to UV- Induced Tumorigenesis Tara I. Ellison, Molly K. Smith, Anita C. Gilliam,

Isothermal Multiple Displacement Amplification

Simultaneous Detection and Quantification of Mitochondrial DNA Deletion(s), Depletion, and Over-Replication in Patients with Mitochondrial Disease Ren-Kui.

Inhibition of UVB-Induced Skin Tumor Development by Drinking Green Tea Polyphenols Is Mediated Through DNA Repair and Subsequent Inhibition of Inflammation

Peroxiredoxin is Ubiquitously Expressed in Rat Skin: Isotype-Specific Expression in the Epidermis and Hair Follicle Jeong Eun Lee, Byung Dae Kwon, Jee-Bum.

Protocol for the Use of Polymerase Chain Reaction in the Detection of Intraocular Large B-Cell Lymphoma in Ocular Samples Aires Lobo, Narciss Okhravi,

Expression of Type XVI Collagen in Human Skin Fibroblasts: Enhanced Expression in Fibrotic Skin Diseases Atsushi Akagi, Shingo Tajima, Yutaka Nagai

Interleukin-17 and Interferon-γ Synergize in the Enhancement of Proinflammatory Cytokine Production by Human Keratinocytes Marcel B.M. Teunissen, Jan.

Molecular Regulation of UVB-Induced Cutaneous Angiogenesis

Radiation Sources Providing Increased UVA/UVB Ratios Attenuate the Apoptotic Effects of the UVB Waveband UVA-Dose-Dependently in Hairless Mouse Skin

Reverse Transcription-3′ Rapid Amplification of cDNA Ends-nested PCR of ACT1 and SAP2 mRNA as a Means of Detecting Viable Candida albicans in an In Vitro.

Implications of Using the ND1 Gene as a Control Region for Real-Time PCR Analysis of Mitochondrial DNA Deletions in Human Skin Andrew Harbottle, Kim.

Active Influx Transport is Mediated by Members of the Organic Anion Transporting Polypeptide Family in Human Epidermal Keratinocytes Ruth Schiffer, Mark.

Type II Collagen Accumulation in Overlying Dermo-Epidermal Junction of Pilomatricoma Is Mediated by Bone Morphogenetic Protein 2 and 4 Hideki Mieno,

Modulation of Collagen Metabolism by the Topical Application of Dehydroepiandrosterone to Human Skin Mi Hee Shin, Gi-eun Rhie, Chi-Hyun Park, Kyu Han.

Naohito Hatta, Craig Dixon, Amanda J. Ray, Sion R. Phillips, William J

Jennifer A. Latimer, James J. Lloyd, Brian L. Diffey, Paul J

Induction of the Photoaging-Associated Mitochondrial Common Deletion In Vivo in Normal Human Skin Mark Berneburg, Heidi Plettenberg, Kathrin Medve-König,

Protein Phosphatase Type 1-Dependent Dephosphorylation of the Retinoblastoma Tumor Suppressor Protein in Ultraviolet-Irradiated Human Skin and Keratinocytes

Responses of Black and White Skin to Solar-Simulating Radiation: Differences in DNA Photodamage, Infiltrating Neutrophils, Proteolytic Enzymes Induced,

H. Randolph Byers, Mina Yaar, Mark S. Eller, Nicole L

Characterization of the CC Chemokine Receptor 3 on Human Keratinocytes

Inhibitory Effect of β-Thujaplicin on Ultraviolet B-Induced Apoptosis in Mouse Keratinocytes Takako Baba, Hajime Nakano, Katsuto Tamai, Daisuke Sawamura,

Transcriptional Regulation of ATP2C1 Gene by Sp1 and YY1 and Reduced Function of its Promoter in Hailey–Hailey Disease Keratinocytes Hiroshi Kawada,

XPA Gene-Deficient, SCF-Transgenic Mice with Epidermal Melanin Are Resistant to UV- Induced Carcinogenesis Fumikazu Yamazaki, Hiroyuki Okamoto, Hiroko.

A Newly Identified Patient with Clinical Xeroderma Pigmentosum Phenotype has a Non- Sense Mutation in the DDB2 Gene and Incomplete Repair in (6-4) Photoproducts

The Melanocortin 5 Receptor is Expressed in Human Sebaceous Glands and Rat Preputial Cells Diane Thiboutot, Aruntha Sivarajah, Kathryn Gilliland, Zhaoyuan.

Protease-Activated Receptor 2, a Receptor Involved in Melanosome Transfer, is Upregulated in Human Skin by Ultraviolet Irradiation Glynis Scott, Cristina.

Identification of Three Species of Borrelia burgdorferi Sensu Lato (B

Expression of μ-Opiate Receptor in Human Epidermis and Keratinocytes

Nature of Mitochondrial DNA Deletions in Substantia Nigra Neurons

Bikunin, a Serine Protease Inhibitor, is Present on the Cell Boundary of Epidermis Cui Chang-Yi, Yoshinori Aragane, Akira Maeda, Piao Yu-Lan, Masae Takahashi,

Syed M. Meeran, Thejass Punathil, Santosh K. Katiyar

Ultraviolet B-Induced Skin Angiogenesis Is Associated with a Switch in the Balance of Vascular Endothelial Growth Factor and Thrombospondin-1 Expression

Carol S. Trempus, John E. French, Raymond W. Tennant

Rab3a and SNARE Proteins: Potential Regulators of Melanosome Movement

The Effect of Thioredoxin on the Expression of Proopiomelanocortin-Derived Peptides, the Melanocortin 1 Receptor and Cell Survival of Normal Human Keratinocytes

Altered Gene Expression in Melanocytes Exposed to 4-Tertiary Butyl Phenol (4-TBP): Upregulation of the A2b Adenosine Receptor1 Fan Yang, Thomas L. Brown,

Variation in Skin Thickness May Explain Some of the Within-Person Variation in Ultraviolet Radiation-Induced Erythema at Different Body Sites Karen Waterston,

Wook Lew Journal of Investigative Dermatology

Kei Wada, Chihaya Maesawa, Toshihide Akasaka, Tomoyuki Masuda

Comparative Analysis of Telomere Lengths and Erosion with Age in Human Epidermis and Lingual Epithelium Ken-Ichi Nakamura, Naotaka Izumiyama-Shimomura,

Post-Transcriptional Regulation of UV Induced TNF-α Expression

The Repair Enzyme Peptide Methionine-S-Sulfoxide Reductase Is Expressed in Human Epidermis and Upregulated by UVA Radiation Fumihide Ogawa, Christina.

RXRα Ablation in Epidermal Keratinocytes Enhances UVR-Induced DNA Damage, Apoptosis, and Proliferation of Keratinocytes and Melanocytes Zhixing Wang,

Diagnosing Treponema pallidum in Secondary Syphilis by PCR and Immunohistochemistry Marc Buffet, Philippe A. Grange, Philippe Gerhardt, Agnès Carlotti,

Expression of Opsin Molecule in Cultured Murine Melanocyte

Neonatal Ultraviolet Radiation Exposure Is Critical for Malignant Melanoma Induction in Pigmented Tpras Transgenic Mice Elke Hacker, Nicole Irwin, H.

Alterations of Glucosylceramide-β-Glucosidase Levels in the Skin of Patients with Psoriasis Vulgaris Francesca Alessandrini, Sabine Pfister, Elisabeth.

Lisa H. Lerner, Abrar A. Qureshi, Bhaskar V. Reddy, Ethan A. Lerner

Immunoprotective UVA (320–400 nm) Irradiation Upregulates Heme Oxygenase-1 in the Dermis and Epidermis of Hairless Mouse Skin Munif Allanson, Vivienne.

Solar-Simulated Ultraviolet Radiation-Induced Upregulation of the Melanocortin-1 Receptor, Proopiomelanocortin, and α-Melanocyte-Stimulating Hormone in.

The Level of Prosaposin is Decreased in the Skin of Patients with Psoriasis Vulgaris Francesca Alessandrini, Silke Stachowitz, Johannes Ring, Heidrun.

Gli1 Protein is Expressed in Basal Cell Carcinomas, Outer Root Sheath Keratinocytes and a Subpopulation of Mesenchymal Cells in Normal Human Skin Lucy.

The Modulation of Aromatase and Estrogen Receptor Alpha in Cultured Human Dermal Papilla Cells by Dexamethasone: A Novel Mechanism for Selective Action.

All-Trans Retinoic Acid Antagonizes UV-Induced VEGF Production and Angiogenesis via the Inhibition of ERK Activation in Human Skin Keratinocytes Mi-Sun.

Keratinocyte Apoptosis Induced by Ultraviolet B Radiation and CD95 Ligation – Differential Protection through Epidermal Growth Factor Receptor Activation.

Evaluation of Apoptotic Cells Induced by Ultraviolet Light B Radiation in Epidermal Sheets Stained by the TUNEL Technique Hiroyuki Okamoto, Kana Mizuno,

Induction of Interleukin-6 Production by Ultraviolet Radiation in Normal Human Epidermal Keratinocytes and in a Human Keratinocyte Cell Line is Mediated.

Giorgio Sirugo, Kenneth K. Kidd The American Journal of Human Genetics

Permeability Barrier Disruption Increases the Level of Serine Palmitoyltransferase in Human Epidermis Francesca Alessandrini, Dr., Heidrun Behrendt

Ultraviolet B Radiation Upregulates the Production of Macrophage Migration Inhibitory Factor (MIF) in Human Epidermal Keratinocytes Tadamichi Shimizu,

Identification of a Commonly Used CDR3 Region of Infiltrating T Cells Expressing Vβ13 and Vβ15 Derived From Psoriasis Patients Ha Young Hwang, Young.

Hepatocyte Growth Factor/Scatter Factor (HGF/SF) Induces Vascular Permeability Factor (VPF/VEGF) Expression by Cultured Keratinocytes Jens Gille, Mona.

Transcriptional Activation of Endogenous Retroviral Sequences in Human Epidermal Keratinocytes by UVB Irradiation Christine Hohenadl, Herbert Germaier,

Presentation transcript:

The Spectrum of Mitochondrial DNA Deletions is a Ubiquitous Marker of Ultraviolet Radiation Exposure in Human Skin Amanda J. Ray, Richard Turner, Osamu Nikaido, Jonathan L. Rees, Mark A. Birch-Machin Journal of Investigative Dermatology Volume 115, Issue 4, Pages 674-679 (October 2000) DOI: 10.1046/j.1523-1747.2000.00092.x Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Increased nuclear staining for thymine dimers in UV-irradiated HaCaT cells. Immunocytochemistry of HaCaT cells with the TDM-2 monoclonal antibody for thymine dimers: (a) nonirradiated; (b) irradiated with 529 mJ per cm2 UVR (290–400 nm). Journal of Investigative Dermatology 2000 115, 674-679DOI: (10.1046/j.1523-1747.2000.00092.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Increased level of thymine dimers in mtDNA purified from UV-irradiated HaCaT cells. Southwestern (a) and southern (b) analyses of mitochondrial DNA purified from HaCaT cells exposed to either 0 or 529 mJ per cm2 of UVR (290–400 nm) (– and +lanes, respectively) and probed (a) with the TDM-2 monoclonal antibody raised thymine dimers and visualized with an enhanced chemiluminescence detection system or (b) a 32P-radiolabeled 470 bp PCR product from the D loop region of the mitochondrial genome (see Materials and Methods). Journal of Investigative Dermatology 2000 115, 674-679DOI: (10.1046/j.1523-1747.2000.00092.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 The mitochondrial origin of the long PCR products were confirmed by southern blot analysis. The spectrum of mtDNA deletions from six samples (lanes 1–3: whole skin samples; lanes 4 and 6: dermal samples; lane 5: epidermal sample) was visualized in the long PCR assay (using primers spanning the 11.1 kb mtDNA region) as a ladder of DNA products on an agarose gel (a). A southern blot of these products was probed with a 32P-radiolabeled 470 bp PCR product from the D loop region of the mitochondrial genome (b). All of the PCR products seen by ethidium bromide staining in (a) were also found to hybridize to the mtDNA probe in (b), confirming that they are derived from the mitochondrial genome. The long PCR assay was validated by the inclusion of several controls that included wild type mtDNA only (thereby amplifying either the 11.1 kb or 5.4 kb product alone) and mtDNA from a Kearns Sayre syndrome patient that harbours 75% of the common deletion (a gift from Professor D. Turnbull, University of Newcastle, U.K.) (results not shown). Journal of Investigative Dermatology 2000 115, 674-679DOI: (10.1046/j.1523-1747.2000.00092.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 A significant increase in the number of deletions with increasing UVR exposure is observed in the epidermis but not the dermis. The number of deletions in (a) epidermal and (b) dermal samples with various levels of UVR exposure. Using the Kruskal–Wallis test, the medians were shown to vary significantly between each exposure group in the epidermis (p =0.0015) but not in the dermis (p =0.6376). Journal of Investigative Dermatology 2000 115, 674-679DOI: (10.1046/j.1523-1747.2000.00092.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 The median age decreases significantly with increasing UVR exposure of the sample groups in the epidermis but not the dermis. The ages of each epidermal (a) and dermal (b) sample are plotted from each UVR exposure group. Using the Kruskal–Wallis test the medians were shown to vary significantly between exposure groups in the epidermis (p =0.0491) but not in the dermis (p =0.7333). Journal of Investigative Dermatology 2000 115, 674-679DOI: (10.1046/j.1523-1747.2000.00092.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions