Hsien-An Pan, M. D. , Yue-Shan Lin, M. D. , Ko-Hung Lee, M. D

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P-24 Fertility and Sterility Volume 85, Pages S19-S20 (April 2006)

Presentation transcript:

Expression patterns of the DAZ-associated protein DAZAP1 in rat and human ovaries Hsien-An Pan, M.D., Yue-Shan Lin, M.D., Ko-Hung Lee, M.D., Jin-Ru Huang, M.Sc., Ying-Hui Lin, M.D., Pao-Lin Kuo, M.D. Fertility and Sterility Volume 84, Pages 1089-1094 (October 2005) DOI: 10.1016/j.fertnstert.2005.03.075 Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 Immunohistochemical staining with anti-DAZAP1 antibodies shows expression of DAZAP1 in the cytoplasm of granulosa luteal (GL) cells from the early stage (A, ×200), mid stage (B, ×200), and late stage (C, ×200) of CL. Early stage of a CL tissue section stained with preimmune serum shows no signals (D, ×200). Pan. Expression patterns of DAZAP1 in ovaries. Fertil Steril 2005. Fertility and Sterility 2005 84, 1089-1094DOI: (10.1016/j.fertnstert.2005.03.075) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 In the rat ovary, immunohistochemical staining shows intense staining of Dazap1 in the cytoplasm of the oocytes (O), theca interna cells (T), and scattered signals in granulosa cells (G) of preovulatory follicles (A, ×100). Immunohistochemical staining of consecutive section with Dazl antibody shows similar staining pattern in rat ovary (B, ×100). Control experiment with preimmune serum shows no signals (C, ×100). There is homogeneous staining of Dazap1 in luteal cells (LC; D, ×100). Consecutive section staining with Dazl antibody shows a similar staining pattern in LC (E, ×100). A control experiment with preimmune serum shows no signals (F, ×100). Pan. Expression patterns of DAZAP1 in ovaries. Fertil Steril 2005. Fertility and Sterility 2005 84, 1089-1094DOI: (10.1016/j.fertnstert.2005.03.075) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 3 Protein extract from rat testis and ovary was incubated with Dazl antibody, and immunoprecipitated proteins were analyzed by Western blot using Dazap1 antibody. Immunoblot analyses of rat testicular and ovarian tissues by using Dazapl antibody were used as positive controls (A). Protein extracts from human testis, CL, and luteinized granulosa cells were incubated with DAZL antibody, and immunoprecipitated proteins were analyzed by Western blot using DAZAP1 antibody. Immunoblot analysis of human testicular tissues by using DAZAP1 antibody was used as a positive control (B). Protein extracts from human testis, CL, and luteinized granulosa cells were incubated with DAZAP1 antibody, and immunoprecipitated proteins were analyzed by Western blot by using DAZL antibody. Immunoblot analysis of human testicular tissues by using DAZL antibody was used as a positive control (B). IB = immunoblot. Pan. Expression patterns of DAZAP1 in ovaries. Fertil Steril 2005. Fertility and Sterility 2005 84, 1089-1094DOI: (10.1016/j.fertnstert.2005.03.075) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 4 Western blot shows decreasing amount of DAZAP1 protein with advancing stages of human CL. Pan. Expression patterns of DAZAP1 in ovaries. Fertil Steril 2005. Fertility and Sterility 2005 84, 1089-1094DOI: (10.1016/j.fertnstert.2005.03.075) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions